Harrie van Erp Rothamsted Research
12 protocols

Marisa Rosa Bio-protocol.org
71 protocols

Matilda Chan University of California
1 protocol

Matthew Escobar California State University San Marcos
1 protocol

Reviewer
Lifeng Liu
  • Post-Doc, DOE Joint Genome Institute
Research focus
  • Molecular biology
  • epigenetics, gene regulation
3 Protocols reviewed
Identification of Intrinsic RNA Binding Specificity of Purified Proteins by in vitro RNA Immunoprecipitation (vitRIP)
Authors:  Marisa Müller, Tamas Schauer and Peter B. Becker, date: 03/05/2021, view: 1187, Q&A: 0

RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized.

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Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
Authors:  Yu Jin and Sebastian Marquardt, date: 10/20/2020, view: 2213, Q&A: 0
CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
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