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A peer-reviewed protocol journal. No publication fee; no access fee.
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Overview
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Harrie van Erp
Rothamsted Research
12 protocols
Marisa Rosa
Bio-protocol.org
70 protocols
Matthew Escobar
California State University San Marcos
1 protocol
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Reviewer
Lifeng Liu
Post-Doc, DOE Joint Genome Institute
Research focus
Plant science
epigenetics, gene regulation
Contributions
Reviewer merit
Editor merit
2 Protocols reviewed
Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable
Cas9-free
Mutants in
Arabidopsis
Authors:
Yu Jin
and
Sebastian Marquardt
,
date:
10/20/2020,
view:
301,
Q&A:
0
CRISPR/Cas9
system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
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Analysis of Xyloglucan Composition in
Arabidopsis
Leaves
Authors:
Javier Sampedro
,
Cristina Gianzo
,
Esteban Guitián
,
Gloria Revilla
and
Ignacio Zarra
,
date:
10/05/2017,
view:
3929,
Q&A:
0
Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species
...
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