Harrie van Erp Rothamsted Research
12 protocols

Marisa Rosa Bio-protocol.org
70 protocols

Matthew Escobar California State University San Marcos
1 protocol

Reviewer
Lifeng Liu
  • Post-Doc, DOE Joint Genome Institute
Research focus
  • Plant science
  • epigenetics, gene regulation
2 Protocols reviewed
Dual sgRNA-based Targeted Deletion of Large Genomic Regions and Isolation of Heritable Cas9-free Mutants in Arabidopsis
Authors:  Yu Jin and Sebastian Marquardt, date: 10/20/2020, view: 141, Q&A: 0
CRISPR/Cas9 system directed by a gene-specific single guide RNA (sgRNA) is an effective tool for genome editing such as deletions of few bases in coding genes. However, targeted deletion of larger regions generate loss-of-function alleles ...
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Analysis of Xyloglucan Composition in Arabidopsis Leaves
Authors:  Javier Sampedro, Cristina Gianzo, Esteban Guitián, Gloria Revilla and Ignacio Zarra, date: 10/05/2017, view: 3901, Q&A: 0
Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species ...
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