Nicole Koropatkin Department of Microbiology and Immunology, University of Michigan Medical School, USA
1 protocol
Dennis Nürnberg Freie Universität Berlin
48 protocols

Gunjan Mehta National Institutes of Health
7 protocols

Joana Antunes Florida International University
3 protocols

Kristin Shingler University of Minnesota School of Dentistry
28 protocols

Reviewer
Darrell Cockburn
  • Assistant professor, Pennsylvania State University
Research focus
  • Microbiology
  • Resistant Starch; Gut Microbiome; Carbohydrate Active Enzymes
  • 1 Author merit
1 Protocol published
Product Analysis of Starch Active Enzymes by TLC
Authors:  Darrell Cockburn and Nicole Koropatkin, date: 10/20/2015, view: 10410, Q&A: 1
Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the ...
8 Protocols reviewed
Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
Authors:  Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty, date: 11/05/2020, view: 1065, Q&A: 0

This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can

...
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Bacterial Synchronized Transfer Assays in Bone Marrow Derived Macrophages
Authors:  Sedelia R Dominguez, Tom Kawula and Shaun P Steele, date: 11/20/2019, view: 2532, Q&A: 0
Merocytophagy (“mero”, Greek for partial; “cytophagy” for cell eating) is a process by which cells acquire microbes and cytosolic material through phagocytosis of a small portion of neighboring cells upon cell-cell contact. Cell-cell contact ...
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