Agnieszka Zienkiewicz Georg August University
13 protocols

Alba Blesa Universidad Francisco de Vitoria
4 protocols

Alberto Rissone National Institutes of Health
2 protocols

Amit Dey Regional Centre for Biotechnology
10 protocols

Editor
David A. Cisneros
  • Research scientist, Umeå University
Research focus
  • Microbiology
  • Microbiology
    Molecular Biology
    Anaerobic microorganisms
    Bacterial Secretion
    CRISPR effectors
    Gut Microbiota
  • 0 Author merit

Education

Dr., TU-Dresden, Germany

Publications

http://scholar.google.com/citations?user=WGDppOUAAAAJ&hl=en

Selected Publications:

Davidson, I. F., Goetz, D., Zaczek, M. P., Molodtsov, M. I., in't Veld, P. J. H., Weissmann, F., Litos, G., Cisneros, D. A., Ocampo-Hafalla, M., Ladurner, R., Uhlmann, F., Vaziri, A. and Peters, J. M. (2016). Rapid movement and transcriptional re-localization of human cohesin on DNA. Embo Journal 35, 2671-2685.

Ladurner, R., Kreidl, E., Ivanov, M. P., Ekker, H., Idarraga-Amado, M. H., Busslinger, G. A., Wutz, G., Cisneros, D. A. and Peters, J. M. (2016) Sororin actively maintains sister chromatid cohesion. Embo Journal 35, pp. 635-653.

Tedeschi, A., Wutz, G., Huet, S.b., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I.F., Tang, W., Cisneros, D.A., Bhaskara, V., Nishiyama T., Vaziri A., Wutz A., Ellenberg J., Peters J.M. (2013). Wapl is an essential regulator of chromatin structure and chromosome segregation. Nature 501, 564-568.

Cisneros, D.A.**, Pehau-Arnaudet, G., and Francetic, O. (2012). Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation. Molecular microbiology 86, 805-818.

Cisneros, D.A.**, Bond, P.J., Pugsley, A.P., Campos, M., and Francetic, O. (2012). Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation. The EMBO journal 31, 1041-1053.

Campos, M., Nilges, M.l., Cisneros, D.A., and Francetic, O. (2010). Detailed structural and assembly model of the type II secretion pilus from sparse data. Proceedings of the National Academy of Sciences 107, 13081-13086.

Cisneros, D.A., Muller, D.J., Daud, S.M., and Lakey, J.H. (2006). An Approach To Prepare Membrane Proteins for Single Molecule Imaging. Angewandte Chemie International Edition 45, 3252-3256.

Cisneros, D.A., Hung, C., Franz, C.M., and Muller, D.J. (2006). Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy. Journal of structural biology 154, 232-245
3 Protocols reviewed
Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
Authors:  Magdiel Martínez, Namyr A. Martínez and Walter I. Silva, date: 07/20/2017, view: 8325, Q&A: 0
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; ...
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Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Authors:  Ran Wang and Sumaira Z. Hasnain, date: 07/20/2017, view: 6346, Q&A: 0
Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 ...
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20 Protocols edited
Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice
Short-Chain Fatty Acids (SCFAs) are a product of the fermentation of resistant starches and dietary fibers by the gut microbiota. The most important SCFA are acetate (C2), propionate (C3) and butyrate (C4). These metabolites are formed and absorbed ...
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Electroporation of Labeled Antibodies to Visualize Endogenous Proteins and Posttranslational Modifications in Living Metazoan Cell Types
Authors:  Sascha Conic, Dominique Desplancq, László Tora and Etienne Weiss, date: 11/05/2018, view: 277, Q&A: 0
The spatiotemporal localization of different intracellular factors in real-time and their detection in live cells are important parameters to understand dynamic protein-based processes. Therefore, there is a demand to perform live-cell imaging and ...
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