Agnieszka Zienkiewicz Nicolaus Copernicus University
21 protocols

Alba Blesa Universidad Francisco de Vitoria
23 protocols

Alberto Rissone National Institutes of Health
3 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
17 protocols

Editor
David A. Cisneros
  • Research scientist, Umeå University
Research focus
  • Microbiology
  • Microbiology
    Molecular Biology
    Anaerobic microorganisms
    Bacterial Secretion
    CRISPR effectors
    Gut Microbiome
  • 0 Author merit

Education

Dr., TU-Dresden, Germany

Lab information

"Molecular biology of human bacterial symbionts"

The intestinal microbiome consists of trillions of beneficial bacteria, which live harmoniously and synergistically with their human hosts. It has only recently been discovered that such microorganisms enormously impact our health in ways that had been completely unanticipated. Because of this, it has become important to understand how this ‘microbiome’ is built and maintained within the intestines, which is where most of our bacteria live. That is the focus of our work.
https://www.cisneros-lab.org/

Publications

http://scholar.google.com/citations?user=WGDppOUAAAAJ&hl=en
Selected Publications:

*Wutz G., *Várnai C., *Nagasaka K. , *Cisneros D.A., Stocsits R.R., Tang W., Schoenfelder S., Jessberger G., Muhar M., Hossain M.J., Walther N., Koch B., Kueblbeck M., Ellenberg J., Zuber J., Fraser P., Peters J.M (2017). Topologically associating domains and chromatin loops depend on cohesin and are regulatedby CTCF, WAPL, and PDS5 proteins. EMBO J. 36(24), 3573-3599.
*SHARED FIRST AUTHOR.

Davidson, I. F., Goetz, D., Zaczek, M. P., Molodtsov, M. I., in't Veld, P. J. H., Weissmann, F., Litos, G., Cisneros, D. A., Ocampo-Hafalla, M., Ladurner, R., Uhlmann, F., Vaziri, A. and Peters, J. M. (2016). Rapid movement and transcriptional re-localization of human cohesin on DNA. Embo Journal 35, 2671-2685.

Ladurner, R., Kreidl, E., Ivanov, M. P., Ekker, H., Idarraga-Amado, M. H., Busslinger, G. A., Wutz, G., Cisneros, D. A. and Peters, J. M. (2016) Sororin actively maintains sister chromatid cohesion. EMBO Journal 35, pp. 635-653.

Tedeschi, A., Wutz, G., Huet, S.b., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I.F., Tang, W., Cisneros, D.A., Bhaskara, V., Nishiyama T., Vaziri A., Wutz A., Ellenberg J., Peters J.M. (2013). Wapl is an essential regulator of chromatin structure and chromosome segregation. Nature 501, 564-568.

Cisneros, D.A., Pehau-Arnaudet, G., and Francetic, O. (2012). Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation. Molecular microbiology 86, 805-818.

Cisneros, D.A., Bond, P.J., Pugsley, A.P., Campos, M., and Francetic, O. (2012). Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation. The EMBO journal 31, 1041-1053.

Campos, M., Nilges, M.l., Cisneros, D.A., and Francetic, O. (2010). Detailed structural and assembly model of the type II secretion pilus from sparse data. Proceedings of the National Academy of Sciences 107, 13081-13086.

Cisneros, D.A., Muller, D.J., Daud, S.M., and Lakey, J.H. (2006). An Approach To Prepare Membrane Proteins for Single Molecule Imaging. Angewandte Chemie International Edition 45, 3252-3256.

Cisneros, D.A., Hung, C., Franz, C.M., and Muller, D.J. (2006). Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy. Journal of structural biology 154, 232-245
4 Protocols reviewed
Assessment of Metacaspase Activity in Phytoplankton
Authors:  Dina Spungin and Ilana Berman-Frank, date: 08/20/2019, view: 2108, Q&A: 0
Programmed cell death (PCD) is an irreversible, genetically-controlled form of cell suicide in which an endogenous biochemical pathway leads to morphological changes and ultimately, cellular demise. PCD is accompanied by de-novo protein ...
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Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
Authors:  Magdiel Martínez, Namyr A. Martínez and Walter I. Silva, date: 07/20/2017, view: 24141, Q&A: 1
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; ...
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29 Protocols edited
Equilibrium and Kinetic Measurements of Ligand Binding to HiBiT-tagged GPCRs on the Surface of Living Cells
Authors:  Michelle E. Boursier, Sergiy Levin, Robin Hurst and Rachel Friedman Ohana, date: 12/20/2020, view: 727, Q&A: 0

G-protein coupled receptors (GPCRs) remain at the forefront of drug discovery efforts. Detailed assessment of features contributing to GPCR ligand engagement in a physiologically relevant environment is imperative to the development of new

...
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In vitro Glutamylation Inhibition of Ubiquitin Modification and Phosphoribosyl-Ubiquitin Ligation Mediated by Legionella pneumophila Effectors
Authors:  Alan G. Sulpizio, Marena E. Minelli and Yuxin Mao, date: 11/05/2020, view: 464, Q&A: 0

Glutamylation is a posttranslational modification where the amino group of a free glutamate amino acid is conjugated to the carboxyl group of a glutamate side chain within a target protein. SidJ is a Legionella kinase-like protein that has recently

...
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