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Coreviewers
Alba Blesa Universidad Francisco de Vitoria
4 protocols

Alberto Rissone National Institutes of Health
2 protocols

Amit Dey Regional Centre for Biotechnology
10 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor
David A. Cisneros
  • Research scientist, Umeå University
Research focus
  • Microbiology
  • Microbiology
    Molecular Biology
    Anaerobic microorganisms
    Bacterial Secretion
    CRISPR effectors
    Gut Microbiota
  • 0 Author merit

Education

Dr., TU-Dresden, Germany

Publications

http://scholar.google.com/citations?user=WGDppOUAAAAJ&hl=en

Selected Publications:

Davidson, I. F., Goetz, D., Zaczek, M. P., Molodtsov, M. I., in't Veld, P. J. H., Weissmann, F., Litos, G., Cisneros, D. A., Ocampo-Hafalla, M., Ladurner, R., Uhlmann, F., Vaziri, A. and Peters, J. M. (2016). Rapid movement and transcriptional re-localization of human cohesin on DNA. Embo Journal 35, 2671-2685.

Ladurner, R., Kreidl, E., Ivanov, M. P., Ekker, H., Idarraga-Amado, M. H., Busslinger, G. A., Wutz, G., Cisneros, D. A. and Peters, J. M. (2016) Sororin actively maintains sister chromatid cohesion. Embo Journal 35, pp. 635-653.

Tedeschi, A., Wutz, G., Huet, S.b., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I.F., Tang, W., Cisneros, D.A., Bhaskara, V., Nishiyama T., Vaziri A., Wutz A., Ellenberg J., Peters J.M. (2013). Wapl is an essential regulator of chromatin structure and chromosome segregation. Nature 501, 564-568.

Cisneros, D.A.**, Pehau-Arnaudet, G., and Francetic, O. (2012). Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation. Molecular microbiology 86, 805-818.

Cisneros, D.A.**, Bond, P.J., Pugsley, A.P., Campos, M., and Francetic, O. (2012). Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation. The EMBO journal 31, 1041-1053.

Campos, M., Nilges, M.l., Cisneros, D.A., and Francetic, O. (2010). Detailed structural and assembly model of the type II secretion pilus from sparse data. Proceedings of the National Academy of Sciences 107, 13081-13086.

Cisneros, D.A., Muller, D.J., Daud, S.M., and Lakey, J.H. (2006). An Approach To Prepare Membrane Proteins for Single Molecule Imaging. Angewandte Chemie International Edition 45, 3252-3256.

Cisneros, D.A., Hung, C., Franz, C.M., and Muller, D.J. (2006). Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy. Journal of structural biology 154, 232-245

3 Protocols reviewed
Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
Authors:  Magdiel Martínez, Namyr A. Martínez and Walter I. Silva, date: 07/20/2017, view: 5779, Q&A: 0
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; ...
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Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Authors:  Ran Wang and Sumaira Z. Hasnain, date: 07/20/2017, view: 4828, Q&A: 0
Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 ...
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14 Protocols edited
In vitro Enzymatic Assays of Histone Decrotonylation on Recombinant Histones
Authors:  Rachel Fellows and Patrick Varga-Weisz, date: 07/20/2018, view: 317, Q&A: 0
Class I histone deacetylases (HDACs) are efficient histone decrotonylases, broadening the enzymatic spectrum of these important (epi-)genome regulators and drug targets. Here, we describe an in vitro approach to assaying class I HDACs with ...
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Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA
Authors:  Christian Reuter and Tobias A. Oelschlaeger, date: 06/20/2018, view: 835, Q&A: 0
The mucosal surfaces of the gastrointestinal, respiratory, reproductive, and urinary tracts, and the surface of the eye harbor a resident microflora that lives in symbiosis with their host and forms a complex ecosystem. The protection of the ...
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