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A peer-reviewed protocol journal. No publication fee; no access fee.
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Beat Bornhauser
Department of Oncology and Children’s Research Center, University Children's Hospital Zurich, Switzerland
1 protocol
Jean-Pierre Bourquin
Department of Oncology and Children’s Research Center, University Children’s Hospital Zurich, Switzerland
1 protocol
Adriano Aguzzi
Institute of Neuropathology, University Hospital of Zurich, Switzerland
1 protocol
Pawel Pelczar
Institute of Laboratory Animal Science, University of Zurich, Switzerland
1 protocol
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Lena Behrmann
Department of Oncology and Children’s Research Center, University Children's Hospital Zurich, Switzerland
Research focus
Molecular biology
Contributions
1
Author merit
Reviewer merit
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1 Protocol published
Efficient Generation of Multi-gene Knockout Cell Lines and Patient-derived Xenografts Using Multi-colored Lenti-CRISPR-Cas9
Authors:
Lena Behrmann
,
Scott McComb
,
Júlia Aguadé-Gorgorió
,
Yun Huang
,
Mario Hermann
,
Pawel Pelczar
,
Adriano Aguzzi
,
Jean-Pierre Bourquin
and
Beat C. Bornhauser
,
date:
04/05/2017,
view:
15358,
Q&A:
0
CRISPR-Cas9 based knockout strategies are increasingly used to analyze gene function. However, redundancies and overlapping functions in biological signaling pathways can call for generating multi-gene knockout cells, which remains a relatively ...
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