Hui Zhang Department of Pathology, University of Michigan, USA
1 protocol

Yali Dou
  • Department of Pathology, University of Michigan, USA
  • 1 Author merit


Ph.D, University of Rochester, Rochester, NY, USA

Current position

Associate Professor of Pathology, Associate Professor of Biological Chemistry, Department of Pathology, University of Michigan Medical School, USA
Associate Professor of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI, USA

Publications (since 2014)

  1. Avedschmidt, S. E., Stagner, A. M., Eagle, R. C., Jr., Harocopos, G. J., Dou, Y. and Rao, R. C. (2016). The Targetable Epigenetic Tumor Protein EZH2 is Enriched in Intraocular Medulloepithelioma. Invest Ophthalmol Vis Sci 57(14): 6242-6246.
  2. Xu, J., Li, L., Xiong, J., denDekker, A., Ye, A., Karatas, H., Liu, L., Wang, H., Qin, Z. S., Wang, S. and Dou, Y. (2016). MLL1 and MLL1 fusion proteins have distinct functions in regulating leukemic transcription program. Cell Discov 2: 16008.
  3. Luo, H., Shenoy, A. K., Li, X., Jin, Y., Jin, L., Cai, Q., Tang, M., Liu, Y., Chen, H., Reisman, D., Wu, L., Seto, E., Qiu, Y., Dou, Y., Casero, R. A., Jr. and Lu, J. (2016). MOF Acetylates the Histone Demethylase LSD1 to Suppress Epithelial-to-Mesenchymal Transition. Cell Rep 15(12): 2665-2678.
  4. Wang, W., Kryczek, I., Dostal, L., Lin, H., Tan, L., Zhao, L., Lu, F., Wei, S., Maj, T., Peng, D., He, G., Vatan, L., Szeliga, W., Kuick, R., Kotarski, J., Tarkowski, R., Dou, Y., Rattan, R., Munkarah, A., Liu, J. R. and Zou, W. (2016). Effector T Cells Abrogate Stroma-Mediated Chemoresistance in Ovarian Cancer. Cell 165(5): 1092-1105.
  5. Zhou, B., Wang, J., Lee, S. Y., Xiong, J., Bhanu, N., Guo, Q., Ma, P., Sun, Y., Rao, R. C., Garcia, B. A., Hess, J. L. and Dou, Y. (2016). PRDM16 Suppresses MLL1r Leukemia via Intrinsic Histone Methyltransferase Activity. Mol Cell.
  6. Zhang, H., Gayen, S., Xiong, J., Zhou, B., Shanmugam, A. K., Sun, Y., Karatas, H., Liu, L., Rao, R. C., Wang, S., Nesvizhskii, A. I., Kalantry, S. and Dou, Y. (2016). MLL1 Inhibition Reprograms Epiblast Stem Cells to Naive Pluripotency. Cell Stem Cell 18(4): 481-494.
  7. Li, Y., Han, J., Zhang, Y., Cao, F., Liu, Z., Li, S., Wu, J., Hu, C., Wang, Y., Shuai, J., Chen, J., Cao, L., Li, D., Shi, P., Tian, C., Zhang, J., Dou, Y., Li, G., Chen, Y. and Lei, M. (2016). Structural basis for activity regulation of MLL family methyltransferases. Nature 530(7591): 447-452.
  8. Tang, H., Fan, X., Xing, J., Liu, Z., Jiang, B., Dou, Y., Gorospe, M. and Wang, W. (2015). NSun2 delays replicative senescence by repressing p27 (KIP1) translation and elevating CDK1 translation. Aging (Albany NY) 7(12): 1143-1158.
  9. Nagarsheth, N., Peng, D., Kryczek, I., Wu, K., Li, W., Zhao, E., Zhao, L., Wei, S., Frankel, T., Vatan, L., Szeliga, W., Dou, Y., Owens, S., Marquez, V., Tao, K., Huang, E., Wang, G. and Zou, W. (2016). PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer. Cancer Res 76(2): 275-282.
  10. Peng, D., Kryczek, I., Nagarsheth, N., Zhao, L., Wei, S., Wang, W., Sun, Y., Zhao, E., Vatan, L., Szeliga, W., Kotarski, J., Tarkowski, R., Dou, Y., Cho, K., Hensley-Alford, S., Munkarah, A., Liu, R. and Zou, W. (2015). Epigenetic silencing of TH1-type chemokines shapes tumour immunity and immunotherapy. Nature 527(7577): 249-253.
  11. Khan, M., Walters, L. L., Li, Q., Thomas, D. G., Miller, J. M., Zhang, Q., Sciallis, A. P., Liu, Y., Dlouhy, B. J., Fort, P. E., Archer, S. M., Demirci, H., Dou, Y. and Rao, R. C. (2015). Characterization and pharmacologic targeting of EZH2, a fetal retinal protein and epigenetic regulator, in human retinoblastoma. Lab Invest 95(11): 1278-1290.
  12. Schaller, M., Ito, T., Allen, R. M., Kroetz, D., Kittan, N., Ptaschinski, C., Cavassani, K., Carson, W. F. t., Godessart, N., Grembecka, J., Cierpicki, T., Dou, Y. and Kunkel, S. L. (2015). Epigenetic regulation of IL-12-dependent T cell proliferation. J Leukoc Biol 98(4): 601-613.
  13. Rao, R. C. and Dou, Y. (2015). Hijacked in cancer: the KMT2 (MLL) family of methyltransferases. Nat Rev Cancer 15(6): 334-346.
  14. Jones, M., Chase, J., Brinkmeier, M., Xu, J., Weinberg, D. N., Schira, J., Friedman, A., Malek, S., Grembecka, J., Cierpicki, T., Dou, Y., Camper, S. A. and Maillard, I. (2015). Ash1l controls quiescence and self-renewal potential in hematopoietic stem cells. J Clin Invest 125(5): 2007-2020.
  15. Lee, Y. T., Gibbons, G., Lee, S. Y., Nikolovska-Coleska, Z. and Dou, Y. (2015). One-pot refolding of core histones from bacterial inclusion bodies allows rapid reconstitution of histone octamer. Protein Expr Purif 110: 89-94.
  16. Komori, H., Xiao, Q., Janssens, D. H., Dou, Y. and Lee, C. Y. (2014). Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor buttonhead. Elife 3.
  17. Yuan, S., Tang, H., Xing, J., Fan, X., Cai, X., Li, Q., Han, P., Luo, Y., Zhang, Z., Jiang, B., Dou, Y., Gorospe, M. and Wang, W. (2014). Methylation by NSun2 represses the levels and function of microRNA 125b. Mol Cell Biol 34(19): 3630-3641.
  18. Benitah, S. A., Bracken, A., Dou, Y., Huangfu, D., Ivanova, N., Koseki, H., Laurent, L., Lim, D. A., Meshorer, E., Pombo, A., Sander, M. and Xu, G. L. (2014). Stem cell epigenetics: looking forward. Cell Stem Cell 14(6): 706-709.
  19. Kryczek, I., Lin, Y., Nagarsheth, N., Peng, D., Zhao, L., Zhao, E., Vatan, L., Szeliga, W., Dou, Y., Owens, S., Zgodzinski, W., Majewski, M., Wallner, G., Fang, J., Huang, E. and Zou, W. (2014). IL-22(+)CD4(+) T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L. Immunity 40(5): 772-784.
  20. Wu, L., Li, L., Zhou, B., Qin, Z. and Dou, Y. (2014). H2B ubiquitylation promotes RNA Pol II processivity via PAF1 and pTEFb. Mol Cell 54(6): 920-931.
  21. Su, X., Zhu, G., Ding, X., Lee, S. Y., Dou, Y., Zhu, B., Wu, W. and Li, H. (2014). Molecular basis underlying histone H3 lysine-arginine methylation pattern readout by Spin/Ssty repeats of Spindlin1. Genes Dev 28(6): 622-636.
  22. Xu, F., Pang, L., Cai, X., Liu, X., Yuan, S., Fan, X., Jiang, B., Zhang, X., Dou, Y., Gorospe, M. and Wang, W. (2014). let-7-repressesed Shc translation delays replicative senescence. Aging Cell 13(1): 185-192.
  23. Cao, F., Townsend, E. C., Karatas, H., Xu, J., Li, L., Lee, S., Liu, L., Chen, Y., Ouillette, P., Zhu, J., Hess, J. L., Atadja, P., Lei, M., Qin, Z. S., Malek, S., Wang, S. and Dou, Y. (2014). Targeting MLL1 H3K4 methyltransferase activity in mixed-lineage leukemia. Mol Cell 53(2): 247-261.
1 Protocol published
Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors
Authors:  Hui Zhang and Yali Dou, date: 03/05/2017, view: 1740, Q&A: 0
Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This ...