José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Adison Wong Singapore Institute of Technology
1 protocol

Beatrice Li University of Oxford
5 protocols

Changyi Zhang Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
3 protocols

Cristina Colomer-Winter Boehringer Ingelheim
1 protocol

Editor
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics molecular biology microorganisms clinical pathogens bacteria yeast
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid, 2016

Lab information

Biotecnología microbiana UFV
José Berenguer's lab: Biotechnology of thermophilic microorganisms
http://www.ufv.es/biotecnologia-microbiana http://web4.cbm.uam.es/joomla-rl/index.php/en/scientific-departments/virology-and-microbiology?id=%20718

Publications

2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 6007, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 5835, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
4 Protocols reviewed
Induction of Natural Competence in Genetically-modified Lactococcus lactis
Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 3065, Q&A: 0
Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence ...
More >
Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli
Authors:  Enrico König, Francesca Zerbini, Ilaria Zanella, Davide Fraccascia and Guido Grandi, date: 01/20/2018, view: 15171, Q&A: 1
With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology since PCR. The key advantage of this method is ...
More >
9 Protocols edited
Rapid Nickel-based Isolation of Extracellular Vesicles from Different Biological Fluids
Extracellular vesicles (EVs) are membranous structures that cells massively release in extracellular fluids. EVs are cargo of cellular components such as lipids, proteins, and nucleic acids that can work as a formidable source in liquid biopsy ...
More >
A Microbial Bioassay for Direct Contact Assessment of Soil Toxicity Based on Oxygen Consumption of Sulfur Oxidizing Bacteria
Authors:  Ebenezer Ashun, Umair Ali Toor, Heonseop Eom and Sang-Eun Oh, date: 01/05/2020, view: 441, Q&A: 0
A new direct contact assessment of soil toxicity using sulfur oxidizing bacteria (SOB) is proposed for analyzing the toxicity of soils. The proposed method is based on the ability of SOB to oxidize elemental sulfur to sulfuric acid in the presence ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.