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Coauthors
José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Coreviewers
Adison Wong Singapore Institute of Technology
1 protocol

David Cisneros Umeå University
20 protocols

Modesto Redrejo-Rodriguez Centro de Biología Molecular Severo Ochoa, Spain
27 protocols

Seda Ekici University of Washington
7 protocols

Reviewer
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics
  • 2 Author merit
2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 3520, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 3650, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
4 Protocols reviewed
Markerless Gene Editing in the Hyperthermophilic Archaeon Thermococcus kodakarensis
Authors:  Alexandra M. Gehring, Travis J. Sanders and Thomas J. Santangelo, date: 11/20/2017, view: 1773, Q&A: 0
The advent of single cell genomics and the continued use of metagenomic profiling in diverse environments has exponentially increased the known diversity of life. The recovered and assembled genomes predict physiology, consortium interactions and ...
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Evaluation of Plasmid Stability by Negative Selection in Gram-negative Bacteria
Authors:  Damián Lobato Márquez and Laura Molina García, date: 05/05/2017, view: 3668, Q&A: 0
Plasmid stability can be measured using antibiotic-resistance plasmid derivatives by positive selection. However, highly stable plasmids are below the sensitivity range of these assays. To solve this problem we describe a novel, highly sensitive ...
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