François Guillemot The Francis Crick Institute, Mill Hill Laboratory, UK
1 protocol
Ehsan Kheradpezhouh Australian National University
9 protocols

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
9 protocols

Longping Tse University of North Carolina at Chapel Hill
24 protocols

Oneil Bhalala Baker Institute
52 protocols

Reviewer
Sébastien Gillotin
  • Research scientist, MSD, Discovery Research MRL
Research focus
  • Neuroscience
  • Neurogenesis, Transcription, Stem Cells, Aging, Metabolism
  • 2 Author merit

Education

Ph.D., Imperial College, London, 2007

Publications

https://www.researchgate.net/profile/Sebastien_Gillotin
  1. Gillotin, S., Davies, J. D. and Philpott, A. (2018). Subcellular localisation modulates ubiquitylation and degradation of Ascl1.Sci Rep 8(1): 4625.  

  2. Gillotin, S. and Guillemot, F. (2016). Micro-chromatin Immunoprecipation(muChIP)Protocol for Real-time PCR Analysis of a Limited Amount of Cells.Bio Protoc 6(12). 

  3. Raposo, A. A., Vasconcelos, F. F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., Bithell, A., Gillotin, S., vanden Berg, D. L., Ettwiller, L., Flicek, P., Crawford, G. E., Parras, C. M., Berninger, B., Buckley, N. J., Guillemot, F. and Castro, D. S. (2015).Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep

  4. Masserdotti, G., Gillotin, S., Sutor, B., Drechsel, D., Irmler, M., Jorgensen, H. F., Sass, S., Theis, F. J., Beckers, J., Berninger, B., Guillemot, F. and Gotz, M. (2015).Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes.Cell Stem Cell 17(1): 74-88. 

  5. Gillotin, S. and Lu, X. (2011).The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.FEBS Lett585(12): 1778-1782. 

  6. Gillotin, S., Yap, D. and Lu, X. (2010).Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation.Cell Cycle 9(7): 1390-1398. 

  7. Gillotin, S. (2009). iASPP,a potential drug target in cancer therapy.Leuk Res 33(9): 1175-1177. 

  8. Slee, E. A., Gillotin, S., Bergamaschi, D., Royer, C., Llanos, S., Ali, S., Jin, B., Trigiante, G. and Lu, X. (2004). The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.Oncogene 23(56): 9007-9016.

2 Protocols published
Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Author:  Sébastien Gillotin, date: 10/05/2018, view: 4368, Q&A: 2
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often ...
Micro-chromatin Immunoprecipitation (μChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Authors:  Sébastien Gillotin and François Guillemot, date: 06/20/2016, view: 4869, Q&A: 0
Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is an important strategy to study gene regulation. When availability of cells is limited, however, it can be useful to focus on specific genes to investigate in depth the role of ...
5 Protocols reviewed
Total RNA Isolation from Separately Established Monolayer and Hydrogel Cultures of Human Glioblastoma Cell Line
Authors:  Manasi P Jogalekar and Elba E Serrano, date: 07/20/2019, view: 760, Q&A: 0
Astrocytoma is an invasive carcinoma occurring in the nervous system and currently lacks effective treatment options. A deeper understanding of the mechanisms of tumorigenesis and tumor progression is needed in order to develop novel therapeutic ...
More >
Primary Embryonic Rat Cortical Neuronal Culture and Chronic Rotenone Treatment in Microfluidic Culture Devices
Authors:  Victor S. Van Laar, Beth Arnold and Sarah B Berman, date: 03/20/2019, view: 1142, Q&A: 0
In the study of neurodegenerative diseases, it is imperative to study the cellular and molecular changes associated with pathogenesis in the relevant cell type, central nervous system neurons. The unique compartmentalized morphology and bioenergetic ...
More >
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.