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Coauthors
François Guillemot The Francis Crick Institute, Mill Hill Laboratory, UK
1 protocol
Coreviewers
Ehsan Kheradpezhouh Australian National University
8 protocols

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
9 protocols

Longping Tse University of North Carolina at Chapel Hill
19 protocols

Oneil Bhalala Baker Institute
44 protocols

Reviewer
Sébastien Gillotin
  • Stem Cell Biologist, Axol Bioscience
Research focus
  • Neuroscience
  • Neurogenesis, Transcription, Stem Cells
  • 2 Author merit

Education

Ph.D., Imperial College, London, 2007

Publications

https://www.researchgate.net/profile/Sebastien_Gillotin
  1. Gillotin, S., Davies, J. D. and Philpott, A. (2018). Subcellular localisation modulates ubiquitylation and degradation of Ascl1.Sci Rep 8(1): 4625.  

  2. Gillotin, S. and Guillemot, F. (2016). Micro-chromatin Immunoprecipation(muChIP)Protocol for Real-time PCR Analysis of a Limited Amount of Cells.Bio Protoc 6(12). 

  3. Raposo, A. A., Vasconcelos, F. F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., Bithell, A., Gillotin, S., vanden Berg, D. L., Ettwiller, L., Flicek, P., Crawford, G. E., Parras, C. M., Berninger, B., Buckley, N. J., Guillemot, F. and Castro, D. S. (2015).Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep

  4. Masserdotti, G., Gillotin, S., Sutor, B., Drechsel, D., Irmler, M., Jorgensen, H. F., Sass, S., Theis, F. J., Beckers, J., Berninger, B., Guillemot, F. and Gotz, M. (2015).Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes.Cell Stem Cell 17(1): 74-88. 

  5. Gillotin, S. and Lu, X. (2011).The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.FEBS Lett585(12): 1778-1782. 

  6. Gillotin, S., Yap, D. and Lu, X. (2010).Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation.Cell Cycle 9(7): 1390-1398. 

  7. Gillotin, S. (2009). iASPP,a potential drug target in cancer therapy.Leuk Res 33(9): 1175-1177. 

  8. Slee, E. A., Gillotin, S., Bergamaschi, D., Royer, C., Llanos, S., Ali, S., Jin, B., Trigiante, G. and Lu, X. (2004). The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.Oncogene 23(56): 9007-9016.

2 Protocols published
Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Author:  Sébastien Gillotin, date: 10/05/2018, view: 138, Q&A: 0
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often ...
Micro-chromatin Immunoprecipitation (μChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Authors:  Sébastien Gillotin and François Guillemot, date: 06/20/2016, view: 3330, Q&A: 0
Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is an important strategy to study gene regulation. When availability of cells is limited, however, it can be useful to focus on specific genes to investigate in depth the role of ...
3 Protocols reviewed
Isolation and Purification of Schwann Cells from Spinal Nerves of Neonatal Rat
Authors:  Jinkun Wen, Dandan Tan, Lixia Li and Jiasong Guo, date: 10/20/2017, view: 3062, Q&A: 0
Primary cultured Schwann cells (SCs) are widely used in the investigation of the biology of SC and are important seed cells for neural tissue engineering. Here, we describe a novel protocol for harvesting primary cultured SCs from neonatal ...
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A High-throughput Assay for mRNA Silencing in Primary Cortical Neurons in vitro with Oligonucleotide Therapeutics
Primary neurons represent an ideal cellular system for the identification of therapeutic oligonucleotides for the treatment of neurodegenerative diseases. However, due to the sensitive nature of primary cells, the transfection of small interfering ...
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