François Guillemot The Francis Crick Institute, Mill Hill Laboratory, UK
1 protocol
Edgar Soria-Gomez Faculty of Medicine and Nursery, University of the Basque Country
25 protocols

Ehsan Kheradpezhouh Australian National University
16 protocols

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
10 protocols

Longping Tse University of North Carolina at Chapel Hill
27 protocols

Reviewer
Sébastien Gillotin
  • Research scientist, MSD, Discovery Research MRL
Research focus
  • Neuroscience
  • Neurogenesis, Transcription, Stem Cells, Aging, Metabolism
  • 2 Author merit

Education

Ph.D., Imperial College, London, 2007

Lab information

https://www.msd-uk.com/research/discovery-centre.xhtml

Publications

https://www.researchgate.net/profile/Sebastien_Gillotin
  1. Gillotin, S., Davies, J. D. and Philpott, A. (2018). Subcellular localisation modulates ubiquitylation and degradation of Ascl1.Sci Rep 8(1): 4625.  

  2. Gillotin, S. and Guillemot, F. (2016). Micro-chromatin Immunoprecipation(muChIP)Protocol for Real-time PCR Analysis of a Limited Amount of Cells.Bio Protoc 6(12). 

  3. Raposo, A. A., Vasconcelos, F. F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., Bithell, A., Gillotin, S., vanden Berg, D. L., Ettwiller, L., Flicek, P., Crawford, G. E., Parras, C. M., Berninger, B., Buckley, N. J., Guillemot, F. and Castro, D. S. (2015).Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep

  4. Masserdotti, G., Gillotin, S., Sutor, B., Drechsel, D., Irmler, M., Jorgensen, H. F., Sass, S., Theis, F. J., Beckers, J., Berninger, B., Guillemot, F. and Gotz, M. (2015).Transcriptional Mechanisms of Proneural Factors and REST in Regulating Neuronal Reprogramming of Astrocytes.Cell Stem Cell 17(1): 74-88. 

  5. Gillotin, S. and Lu, X. (2011).The ASPP proteins complex and cooperate with p300 to modulate the transcriptional activity of p53.FEBS Lett585(12): 1778-1782. 

  6. Gillotin, S., Yap, D. and Lu, X. (2010).Mutation at Ser392 specifically sensitizes mutant p53H175 to mdm2-mediated degradation.Cell Cycle 9(7): 1390-1398. 

  7. Gillotin, S. (2009). iASPP,a potential drug target in cancer therapy.Leuk Res 33(9): 1175-1177. 

  8. Slee, E. A., Gillotin, S., Bergamaschi, D., Royer, C., Llanos, S., Ali, S., Jin, B., Trigiante, G. and Lu, X. (2004). The N-terminus of a novel isoform of human iASPP is required for its cytoplasmic localization.Oncogene 23(56): 9007-9016.

2 Protocols published
Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Author:  Sébastien Gillotin, date: 10/05/2018, view: 9858, Q&A: 3
Shuttling of proteins between different cellular compartments controls their proteostasis and can contribute in some cases to regulate their activity. Biochemical analysis of chromatin-bound proteins, such as transcription factors, is often ...
Micro-chromatin Immunoprecipitation (μChIP) Protocol for Real-time PCR Analysis of a Limited Amount of Cells
Authors:  Sébastien Gillotin and François Guillemot, date: 06/20/2016, view: 6098, Q&A: 0
Chromatin immunoprecipitation followed by deep sequencing (ChIP-Seq) is an important strategy to study gene regulation. When availability of cells is limited, however, it can be useful to focus on specific genes to investigate in depth the role of ...
9 Protocols reviewed
Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation
The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of ...
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Flow Cytometry Measurement of Glucocerebrosidase Activity in Human Monocytes
Authors:  Laura P. Hughes, Glenda M. Halliday and Nicolas Dzamko, date: 04/05/2020, view: 1290, Q&A: 0
Glucocerebrosidase (GCase) is an important enzyme for the metabolism of glycolipids. GCase enzyme deficiency is implicated in human disease and the efficient measurement of GCase activity is important for evaluating the efficacy of therapeutics ...
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