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Amy Kiger Division of Biological Sciences, University of California, USA
2 protocols
Chunjing Qu Baylor Genetics Laboratories/Baylor College of Medicine
2 protocols

HongLok Lung University of Hong Kong
58 protocols

Jihyun Kim Finless Foods
19 protocols

Reviewer
Steve Jean
  • Faculty, Université de Sherbrooke
Research focus
  • Cell biology
  • Membrane trafficking, Autophagy, Drosophila
  • 2 Author merit

Education

Ph.D., Université Laval, 2008

Lab information

https://www.usherbrooke.ca/dep-anatomie-biologie-cellulaire/personnel/professeurs-reguliers/jean-steve/

Publications

Peer-Reviewed articles:
5- Jean S., Mikryukov A., Tremblay MG., Baril J., Guillou F., Bellenfant S., Moss T. (2010). Extended-Synaptotagmin-2 mediates FGF Receptor endocytosis and ERK activation in vivo. Developmental Cell, Sep 14;19(3):426-39.

6- Jean S., Tremblay MG., Herdman C., Guillou F., Moss T. (2012). The endocytic adapter E-Syt2 recruits the p21 GTPase activated kinase PAK1 to mediate actin dynamics and FGF signalling. Biology Open. Aug 15;(1): 731-738.

7- Jean S., Cox S., Schmidt EJ., Robinson FL., Kiger AA. (2012). Sbf/MTMR13 coordinates PI(3)P and Rab21 regulation in endocytic control of cellular remodeling. Molecular Biology of the Cell, Jul;23(14):2723-40.

8- Jean S. and Kiger AA. (2012). Coordination between Rab GTPase and phosphoinositide regulation and functions. Nature Reviews Molecular Cell Biology, Jun 22;13(7): 463-470.

9- Jean S. and Kiger AA. (2014). Classes of phosphoinositide 3-kinases at a glance. Journal of Cell Science, Mar 1;127 (Pt 5): 923-928.

10- Jean S., Cox S., Nassari S. and Kiger AA. (2015). Starvation-induced MTMR13 and RAB21 activity regulates VAMP8 to promote autophagosome-lysosome fusion. EMBO Reports, 16(3): 297-311.

11- Klionsky, D.J. et al., including Jean, S. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12, 1-222.

12- Jean S#. and Kiger AA#. (2016). VAMP8-3xHA uptake assays in HeLa cells. Bio-Protocol. Feb. Vol 6, Issue 4. #Indicates co-corresponding authors.

13- Jean S#. and Kiger AA#. (2016). RAB21 activity measurement using GST-fused APPL1. Bio-Protocol. Feb. Vol 6, Issue 4. #Indicates co-corresponding authors.

14- Fujita N., Huang W., Lin TH., Groulx JF., Jean S., Kuchitsu Y., Koyama-Honda I., Mizushima N., Fukuda M., Kiger AA. (2017). Genetic screen in Drosophila muscle identifies autophagy-mediated T-tubule remodeling and a Rab2 role in autophagy. Elife. 2017 Jan 7;6. pii: e23367.

15- Paquette M., Beaudoin S., Tremblay MA., Jean S., Lopez A., Lecomte R., Guérin B., Bentourkia M., Sabbagh R., Leyton J. NLS-cholic acid conjugation to IL-5Rα-specific antibody improves cellular accumulation and in vivo tumor-targeting properties in a bladder cancer model. Bioconjugate Chemistry. In press

Book Chapter:
1- Lauzier A, Jean S. (2018) Autophagic flux assessment in colorectal cancer cells. Methods Molecular Biology, Vol. 1765, Colorectal Cancer, 978-1-4939-7764-2
2 Protocols published
VAMP8-3xHA Uptake Assay in HeLa Cells
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 3478, Q&A: 0
Transmembrane proteins are rarely exclusively localized to a specific vesicle or an organelle. Most transmembrane proteins undergo complicated trafficking routes. Thus, transmembrane proteins are under constant flux, and at steady state, found on a ...
RAB21 Activity Assay Using GST-fused APPL1
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 3502, Q&A: 0
The Rab family of small GTPases are essential regulators of membrane trafficking events. As with other small GTPase families, Rab GTPases cycle between an inactive GDP- bound state and an active GTP-bound state. Guanine nucleotide exchange factors ...
2 Protocols reviewed
Synthetic Lethality Screens Using RNAi in Combination with CRISPR-based Knockout in Drosophila Cells
Authors:  Benjamin E. Housden, Hilary E. Nicholson and Norbert Perrimon, date: 02/05/2017, view: 4115, Q&A: 0
A synthetic lethal interaction is a type of genetic interaction where the disruption of either of two genes individually has little effect but their combined disruption is lethal. Knowledge of synthetic lethal interactions can allow for elucidation ...
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Lentiviral shRNA Screen to Identify Epithelial Integrity Regulating Genes in MCF10A 3D Culture
Authors:  Elsa Marques and Juha Klefström, date: 12/05/2016, view: 4070, Q&A: 0
MCF10A 3D culture system provides a reductionist model of glandular mammary epithelium which is widely used to study development of glandular architecture, the role of cell polarity and epithelial integrity in control of epithelial cell functions, ...
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