Vasudevan Achuthan —BIO-PROTOCOL

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Jeffrey DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
41 protocols

Arsalan Daudi University of California
157 protocols

Chhuttan Meena National Chemical laboratory NCL Pune
2 protocols

Luis Alberto Vargas Institute for Immunology and Informatics. University of Rhode Island
8 protocols

Reviewer

Vasudevan Achuthan

Post-Doc, Dana Farber Cancer Institute

Research focus

Microbiology
HIV integration and latency

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Maryland, 2016

Lab information

PI: Alan Engelman

Publications

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.

2 Protocols published

Primer Extension Reactions for the PCR- based α- complementation Assay

Authors: Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 8287, Q&A: 0

The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...

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Mismatched Primer Extension Assays

Authors: Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 5783, Q&A: 0

Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...

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7 Protocols reviewed

Evaluation of the Sequence Variability within the PCR Primer/Probe Target Regions of the SARS-CoV-2 Genome

Authors: Kashif Aziz Khan and Peter Cheung, date: 12/20/2020, view: 903, Q&A: 0

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; initially named 2019-nCoV) is responsible for the recent coronavirus disease (COVID-19) pandemic, and polymerase chain reaction (PCR) is the current standard method for diagnosis from ...

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Spectrophotometric Assessment of Heme Oxygenase-1 Activity in Leishmania-infected Macrophages

Authors: Moumita Basu, Shriya Saha and Anindita Ukil, date: 04/05/2020, view: 1529, Q&A: 0

Heme oxygenase-1 (HO-1) is a stress responsive enzyme that metabolizes heme and releases free iron, carbon monoxide (CO), and biliverdin (BV), which rapidly undergoes conversion to bilirubin (BL). Estimation of bilirubin is the basis of HO-1 assay. ...

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