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Coauthors
Jeffrey DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols
Coreviewers
Arsalan Daudi University of California
157 protocols

Modesto Redrejo-Rodriguez Centro de Biología Molecular Severo Ochoa, Spain
26 protocols

Pooja Saxena Medicago R&D Inc.
5 protocols

Yannick Debing Janssen Pharmaceutica
27 protocols

Reviewer
Vasudevan Achuthan
  • Post-Doc, Dana Farber Cancer Institute
Research focus
  • Microbiology
  • HIV integration and latency
  • 2 Author merit

Education

PhD, University of Maryland, 2016

Lab information

PI: Alan Engelman

Publications

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.
2 Protocols published
Primer Extension Reactions for the PCR- based α- complementation Assay
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 4129, Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
Mismatched Primer Extension Assays
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 3228, Q&A: 0
Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...
3 Protocols reviewed
Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
Authors:  Jin-Wei Zheng, Jia-Li Cao and Quan Yuan , date: 07/05/2018, view: 495, Q&A: 0
The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to ...
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Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins
Authors:  Xu Zheng and David Pincus, date: 06/20/2017, view: 2614, Q&A: 0
This method was generated to isolate high affinity protein complexes from yeast lysate by performing serial affinity purification of doubly tagged 3xFLAG/V5 proteins. First, the bait protein of interest is immunoprecipitated by anti-FLAG-conjugated ...
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