Vasudevan Achuthan —BIO-PROTOCOL

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Jeffrey DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
26 protocols

Arsalan Daudi University of California
157 protocols

Luis Alberto Vargas Institute for Immunology and Informatics. University of Rhode Island
4 protocols

Miao He Institute of Brain Science, Fudan University
6 protocols

Reviewer

Vasudevan Achuthan

Post-Doc, Dana Farber Cancer Institute

Research focus

Microbiology
HIV integration and latency

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University of Maryland, 2016

Lab information

PI: Alan Engelman

Publications

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.

2 Protocols published

Primer Extension Reactions for the PCR- based α- complementation Assay

Authors: Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 7143, Q&A: 0

The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...

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Mismatched Primer Extension Assays

Authors: Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 5123, Q&A: 0

Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...

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4 Protocols reviewed

Separation of Natural Collagen Crosslinks Using Buffer and Ion-pairing Agent Free Solvents on Silica Hydride Column for Mass Spectrometry Detection

Authors: Rafea Naffa and Joseph Pesek, date: 05/05/2019, view: 1184, Q&A: 0

In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column ...

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Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines

Authors: Jin-Wei Zheng, Jia-Li Cao and Quan Yuan , date: 07/05/2018, view: 3598, Q&A: 0

The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to ...

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