Jeffrey DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols
Alexandros Alexandratos National and Kapodistrian University of Athens
30 protocols

Arsalan Daudi University of California
157 protocols

Luis Alberto Vargas Institute for Immunology and Informatics. University of Rhode Island
6 protocols

Miao He Institute of Brain Science, Fudan University
8 protocols

Reviewer
Vasudevan Achuthan
  • Post-Doc, Dana Farber Cancer Institute
Research focus
  • Microbiology
  • HIV integration and latency
  • 2 Author merit

Education

PhD, University of Maryland, 2016

Lab information

PI: Alan Engelman

Publications

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.
2 Protocols published
Primer Extension Reactions for the PCR- based α- complementation Assay
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 7528, Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
Mismatched Primer Extension Assays
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 5340, Q&A: 0
Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...
6 Protocols reviewed
Spectrophotometric Assessment of Heme Oxygenase-1 Activity in Leishmania-infected Macrophages
Authors:  Moumita Basu, Shriya Saha and Anindita Ukil, date: 04/05/2020, view: 599, Q&A: 0
Heme oxygenase-1 (HO-1) is a stress responsive enzyme that metabolizes heme and releases free iron, carbon monoxide (CO), and biliverdin (BV), which rapidly undergoes conversion to bilirubin (BL). Estimation of bilirubin is the basis of HO-1 assay. ...
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Preparation of a Bacteriophage T4-based Prokaryotic-eukaryotic Hybrid Viral Vector for Delivery of Large Cargos of Genes and Proteins into Human Cells
Authors:  Jingen Zhu, Pan Tao, Marthandan Mahalingam and Venigalla B. Rao, date: 04/05/2020, view: 491, Q&A: 0
A viral vector that can safely and efficiently deliver large and diverse molecular cargos into cells is the holy grail of curing many human diseases. Adeno-associated virus (AAV) has been extensively used but has a very small capacity. The ...
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