Jeffrey DeStefano Department of Cell Biology and Molecular Genetics, University of Maryland, USA
2 protocols
Alessandro Didonna University of California, San Francisco
51 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
51 protocols

Arsalan Daudi University of California
157 protocols

Chhuttan Meena National Chemical laboratory NCL Pune
3 protocols

Reviewer
Vasudevan Achuthan
  • Post-Doc, Dana Farber Cancer Institute
Research focus
  • Microbiology
  • HIV integration and latency
  • 2 Author merit

Education

PhD, University of Maryland, 2016

Lab information

PI: Alan Engelman

Publications

1. Achuthan V, Keith BJ, Connolly BA, DeStefano JJ., 2014, Human immunodeficiency virus reverse transcriptase displays dramatically higher fidelity under physiological magnesium conditions in vitro, J.Virol;88(15):8514-27, PMCID: PMC4135932.
2. Achuthan V, DeStefano JJ., 2015, Alternative divalent cations (Zn2+, Co2+, and Mn2+) are not mutagenic at conditions optimal for reverse transcriptase activity, BMC Biochemistry; May 3;16(1):12, PMCID: PMC4472245.
3. Achuthan V, DeStefano JJ., 2015, Primer extension reactions for the PCR-based lacZα complementation fidelity assay, Bio-protocol, 5(12): e1509, PMCID: PMC4972330.
4. Achuthan V, DeStefano JJ., 2015, Mismatched primer extension assays, Bio-protocol, 5(12): e1508, PMCID: PMC4829118.
5. Achuthan V, Singh K, DeStefano JJ., 2017, Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro, Biochemistry; 2017 Jan 10;56(1):33-46, PMCID: PMC5453313.
2 Protocols published
Primer Extension Reactions for the PCR- based α- complementation Assay
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 9055, Q&A: 0
The PCR- based- α- complementation assay is an effective technique to measure the fidelity of polymerases, especially RNA-dependent RNA polymerases (RDRP) and Reverse Transcriptases (RT). It has been successfully employed to determine the fidelity ...
Mismatched Primer Extension Assays
Authors:  Vasudevan Achuthan and Jeffrey J. DeStefano, date: 06/20/2015, view: 6313, Q&A: 0
Steady state kinetic assays have been a reliable way to estimate fidelity of several polymerases (Menendez-Arias, 2009; Rezende and Prasad, 2004; Svarovskaia et al., 2003). The ability to analyze the extension of primers with specific ...
9 Protocols reviewed
Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
Author:  Mansoor A. Siddiqui, date: 05/05/2021, view: 2337, Q&A: 0

Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases

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A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
Authors:  Benno Zehnder, Stephan Urban and Thomas Tu, date: 04/20/2021, view: 2947, Q&A: 0

Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral

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