Akira Karasawa Molecular Medicine, Cornell University, USA
5 protocols

Alexandros Kokotos Weill Cornell Medicine
11 protocols

Alka Mehra Institute of Genomics and Integrative Biology
31 protocols

Aruna Bitra La Jolla institute for allergy and immunology
2 protocols

Editor
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 8070, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
4 Protocols reviewed
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Authors:  Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 4750, Q&A: 0
Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...
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39 Protocols edited
Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol
Authors:  Xue Han, Keigo Yoshizaki, Tian Tian, Kanako Miyazaki, Ichiro Takahashi and Satoshi Fukumoto, date: 02/05/2020, view: 129, Q&A: 0
A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and ...
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Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
Authors:  W. Michael Babinchak and Witold K. Surewicz, date: 01/20/2020, view: 434, Q&A: 0
Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative ...
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