Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Alexandros Kokotos University of Edinburgh
4 protocols

Alka Mehra Institute of Genomics and Integrative Biology
21 protocols

Chris Tibbitt Karolinska Institutet
3 protocols

Editor
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 6416, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
4 Protocols reviewed
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Authors:  Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 3283, Q&A: 0
Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...
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31 Protocols edited
Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes
Authors:  Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson, date: 12/05/2018, view: 328, Q&A: 0
To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type ...
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Relative Quantitation of Polymerized Actin in Suspension Cells by Flow Cytometry
Authors:  Mallory R. Kakley, Katrina B. Velle and Lillian K. Fritz-Laylin, date: 11/20/2018, view: 496, Q&A: 0
The amount of polymerized actin within a cell can vary widely due to natural processes and/or experimentally induced perturbations. We routinely use this protocol to measure relative polymerized actin content between cell populations by staining ...
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