Akira Karasawa New York Structural Biology Center
8 protocols

Alba Blesa Universidad Francisco de Vitoria
26 protocols

Alexandros Kokotos Weill Cornell Medicine
18 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Editor
Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit
1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 9667, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
6 Protocols reviewed
Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
Authors:  Rezwan Siddiquee and Ann H Kwan, date: 05/20/2021, view: 2113, Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using

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44 Protocols edited
Preparation of Human Chondrocytes for Profiling Using Cytometry by Time-of-flight (cyTOF)
Authors:  Fiorella Carla Grandi and Nidhi Bhutani, date: 07/20/2021, view: 757, Q&A: 0

Single-cell technologies have allowed high-resolution profiling of tissues and thus a deeper understanding of tissue homeostasis and disease heterogeneity. Understanding this heterogeneity can be especially important for tailoring treatments in a

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Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
Authors:  Henrik Renner, Mandy Otto, Martha Grabos, Hans R. Schöler and Jan M. Bruder, date: 06/20/2021, view: 1130, Q&A: 1

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically,

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