Alka Mehra Institute of Genomics and Integrative Biology
15 protocols

Chris Tibbitt Karolinska Institutet
1 protocol

Cody Kime Center of Developmental Biology, RIKEN
1 protocol

Cristina Rohena University of California, San Francisco
1 protocol

Ralph Thomas Boettcher
  • Project group leader, Max Planck Institute for Biochemistry
Research focus
  • Cell biology
  • 1 Author merit


1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:  Ralph T. Böttcher, date: 11/05/2013, view: 5090, Q&A: 0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
4 Protocols reviewed
Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
More >
VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells
Authors:  Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 2067, Q&A: 0
Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...
More >
21 Protocols edited
RAB21 Activity Assay Using GST-fused APPL1
Authors:  Steve Jean and Amy A. Kiger, date: 02/20/2016, view: 2656, Q&A: 0
The Rab family of small GTPases are essential regulators of membrane trafficking events. As with other small GTPase families, Rab GTPases cycle between an inactive GDP- bound state and an active GTP-bound state. Guanine nucleotide exchange factors ...
More >
Culture of Megakaryocytes from Human Peripheral Blood Mononuclear Cells
Authors:  Vishal Salunkhe, Petros Papadopoulos and Laura Gutiérrez, date: 11/05/2015, view: 8923, Q&A: 0
Megakaryocytes are the precursor cells of platelets and are bona fide resident cells in the bone marrow but extremely low in numbers (~1% of total nucleated cells). Upon terminal differentiation, megakaryocytes increase their size, become ...
More >