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Garrett Berry Gene Therapy Center, The University of North Carolina at Chapel Hill, USA
1 protocol

Gary Whittaker Department of Microbiology and Immunology, Cornell University, United States
2 protocols

Yueting Zhang Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, USA
1 protocol
Arsalan Daudi University of California
157 protocols

Daan Swarts Department of Biochemistry, University of Zurich, Switzerland
9 protocols

David Paul Medical Research Council
12 protocols

Ehsan Kheradpezhouh Australian National University
8 protocols

Editor
Longping Victor Tse
  • Post-Doc, University of North Carolina at Chapel Hill
Research focus
  • Gene Therapy
  • Gene Delivery
    Vector Engineering
    Infectious Diseases
  • 2 Author merit

Education

PhD, Cornell University, 2014

Publications

2 Protocols published
Virus Binding and Internalization Assay for Adeno-associated Virus
Authors:  Garrett E. Berry and Longping V. Tse, date: 01/20/2017, view: 4192, Q&A: 0
The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight ...
Immunoplaque Assay (Influenza Virus)
Authors:  Longping V. Tse, Yueting Zhang and Gary R. Whittaker, date: 11/05/2013, view: 11835, Q&A: 0
Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the ...
5 Protocols reviewed
Expression and Purification of the Cas10-Csm Complex from Staphylococci
Authors:  Lucy Chou-Zheng and Asma Hatoum-Aslan, date: 06/05/2017, view: 3560, Q&A: 0
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a class of prokaryotic immune systems that degrade foreign nucleic acids in a sequence-specific manner. These systems rely upon ribonucleoprotein ...
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Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 3745, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...
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15 Protocols edited
Quantification of Infectious Sendai Virus Using Plaque Assay
Authors:  Narihito Tatsumoto, Takamasa Miyauchi, Moshe Arditi and Michifumi Yamashita, date: 11/05/2018, view: 75, Q&A: 0
Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy. Virus ...
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Detection and Differentiation of Multiple Viral RNAs Using Branched DNA FISH Coupled to Confocal Microscopy and Flow Cytometry
Authors:  Nicholas van Buuren and Karla Kirkegaard, date: 10/20/2018, view: 303, Q&A: 0
Due to the exceptionally high mutation rates of RNA-dependent RNA polymerases, infectious RNA viruses generate extensive sequence diversity, leading to some of the lowest barriers to the development of antiviral drug resistance in the microbial ...
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