Garrett Berry Gene Therapy Center, The University of North Carolina at Chapel Hill, USA
1 protocol

Gary Whittaker Department of Microbiology and Immunology, Cornell University, United States
2 protocols

Yueting Zhang Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, USA
1 protocol
Akira Karasawa Molecular Medicine, Cornell University, USA
5 protocols

Arsalan Daudi University of California
157 protocols

Beatrice Li University of Oxford
5 protocols

Christina Tan Agency for Science, Technology and Research (A*STAR)
2 protocols

Editor
Longping Victor Tse
  • Post-Doc, University of North Carolina at Chapel Hill
Research focus
  • Gene Therapy
  • Gene Delivery
    Vector Engineering
    Infectious Diseases
  • 2 Author merit

Education

PhD, Cornell University, 2014

Publications

2 Protocols published
Virus Binding and Internalization Assay for Adeno-associated Virus
Authors:  Garrett E. Berry and Longping V. Tse, date: 01/20/2017, view: 6996, Q&A: 0
The binding and internalization of adeno-associated virus (AAV) is an important determinant of viral infectivity and tropism. The ability to dissect these two tightly connected cellular processes would allow better understanding and provide insight ...
Immunoplaque Assay (Influenza Virus)
Authors:  Longping V. Tse, Yueting Zhang and Gary R. Whittaker, date: 11/05/2013, view: 16599, Q&A: 1
Despite developed long time ago, plaque assay is still the gold standard for viral titer quantification in modern virology. The standard crystal violet-based plaque assay relies on virus’ ability to induce cytopathic effect (CPE) which limits the ...
5 Protocols reviewed
Expression and Purification of the Cas10-Csm Complex from Staphylococci
Authors:  Lucy Chou-Zheng and Asma Hatoum-Aslan, date: 06/05/2017, view: 5964, Q&A: 0
CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a class of prokaryotic immune systems that degrade foreign nucleic acids in a sequence-specific manner. These systems rely upon ribonucleoprotein ...
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Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors
Authors:  Chin-kai Chuang, Ching-Fu Tu and Chien-Hong Chen, date: 06/05/2017, view: 6320, Q&A: 0
A set of Cas9 and single guide CRISPR RNA expression vectors was constructed. Only a very simple procedure was needed to prepare specific single-guide RNA expression vectors with high target accuracy. Since the de novo zygotic transcription ...
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21 Protocols edited
Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli
Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for ...
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Localizing Genome Segments and Protein Products of a Multipartite Virus in Host Plant Cells
Authors:  Marie-Stephanie Vernerey, Elodie Pirolles, Stephane Blanc and Anne Sicard, date: 12/05/2019, view: 542, Q&A: 0
A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. This concept applied to multipartite viruses–which have a genome composed of two or more nucleic acid segments, each individually ...
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