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Alizée Malnoë University of California, Berkeley
3 protocols

Ana Espinosa-Ruiz Instituto de Biología Molecular y Celular de Plantas
1 protocol

Annis Richardson University of California, Berkeley
4 protocols

Arsalan Daudi University of California
153 protocols

Editor
Renate Weizbauer
  • Post-Doc, Carnegie Institution for Science
Research focus
  • Plant science
  • 0 Author merit

Education

Ph.D., Purdue University

14 Protocols reviewed
Plant Tissue Trypan Blue Staining During Phytopathogen Infection
In this protocol plant tissue is stained with trypan blue dye allowing the researcher to visualize cell death. Specifically this method avoids the use of the carcinogen compound chloral hydrate, making this classical method of staining safer and ...
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A Ribosome Footprinting Protocol for Plants
Authors:  Catharina Merchante, Qiwen Hu, Steffen Heber, Jose Alonso and Anna N. Stepanova, date: 11/05/2016, view: 2957, Q&A: 0
Ribosome footprinting, or Ribo-seq, has revolutionized the studies of translation. It was originally developed for yeast and mammalian cells in culture (Ingolia et al., 2009). Herein, we describe a plant-optimized hands-on ribosome ...
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19 Protocols edited
Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
Authors:  Akiteru Maeno and Katsutoshi Tsuda, date: 01/05/2018, view: 189, Q&A: 0
Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular ...
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A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 502, Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
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