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Coauthors
René van der Ploeg Hogeschool Utrecht, Hogeschool Utrecht, The Netherlands, The Netherlands,
1 protocol

Svetlana Alexeeva Laboratory of Food Microbiology, Wageningen University, The Netherlands, The Netherlands,
1 protocol

Nils Meiresonne Bacterial Cell Biology & Physiology, University of Amsterdam, The Netherlands, The Netherlands,
1 protocol

Nienke Buddelmeijer Institut Pasteur, Biology and Genetics of the Bacterial Cell Wall Unit, INSERM Group Avenir, France
2 protocols

Tanneke den Blaauwen
  • Bacterial Cell Biology & Physiology, University of Amsterdam, The Netherlands, The Netherlands,
  • 2 Author merit

Education

Ph.D.in Biology, Department of the University of Amsterdam, 1988

Current position

Associate professor University of Amsterdam, Amsterdam Faculty of Science, Bacterial Cell Biology

Publications (since 2005)

  1. Meiresonne, N. Y., van der Ploeg, R., Hink, M. A. and den Blaauwen, T. (2017). Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli. MBio 8(5).
  2. den Blaauwen, T., Hamoen, L. W. and Levin, P. A. (2017). The divisome at 25: the road ahead. Curr Opin Microbiol 36: 85-94.
  3. Leisch, N., Pende, N., Weber, P. M., Gruber-Vodicka, H. R., Verheul, J., Vischer, N. O., Abby, S. S., Geier, B., den Blaauwen, T. and Bulgheresi, S. (2016). Asynchronous division by non-ring FtsZ in the gammaproteobacterial symbiont of Robbea hypermnestra. Nat Microbiol 2: 16182.
  4. Hugonnet, J. E., Mengin-Lecreulx, D., Monton, A., den Blaauwen, T., Carbonnelle, E., Veckerle, C., Brun, Y. V., van Nieuwenhze, M., Bouchier, C., Tu, K., Rice, L. B. and Arthur, M. (2016). Factors essential for L,D-transpeptidase-mediated peptidoglycan cross-linking and beta-lactam resistance in Escherichia coli. Elife 5.
  5. Glas, M., van den Berg van Saparoea, H. B., McLaughlin, S. H., Roseboom, W., Liu, F., Koningstein, G. M., Fish, A., den Blaauwen, T., Heck, A. J., de Jong, L., Bitter, W., de Esch, I. J. and Luirink, J. (2015). The Soluble Periplasmic Domains of Escherichia coli Cell Division Proteins FtsQ/FtsB/FtsL Form a Trimeric Complex with Submicromolar Affinity. J Biol Chem 290(35): 21498-21509.
  6. van der Ploeg, R., Goudelis, S. T. and den Blaauwen, T. (2015). Validation of FRET Assay for the Screening of Growth Inhibitors of Escherichia coli Reveals Elongasome Assembly Dynamics. Int J Mol Sci 16(8): 17637-17654.
  7. Vischer, N. O., Verheul, J., Postma, M., van den Berg van Saparoea, B., Galli, E., Natale, P., Gerdes, K., Luirink, J., Vollmer, W., Vicente, M. and den Blaauwen, T. (2015). Cell age dependent concentration of Escherichia coli divisome proteins analyzed with ImageJ and ObjectJ. Front Microbiol 6: 586.
  8. Gray, A. N., Egan, A. J., Van't Veer, I. L., Verheul, J., Colavin, A., Koumoutsi, A., Biboy, J., Altelaar, A. F., Damen, M. J., Huang, K. C., Simorre, J. P., Breukink, E., den Blaauwen, T., Typas, A., Gross, C. A. and Vollmer, W. (2015). Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division. Elife 4.
  9. Pende, N., Leisch, N., Gruber-Vodicka, H. R., Heindl, N. R., Ott, J., den Blaauwen, T. and Bulgheresi, S. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun 5: 4803.
  10. den Blaauwen, T., Andreu, J. M. and Monasterio, O. (2014). Bacterial cell division proteins as antibiotic targets. Bioorg Chem 55: 27-38.
  11. Mohammadi, T., Sijbrandi, R., Lutters, M., Verheul, J., Martin, N. I., den Blaauwen, T., de Kruijff, B. and Breukink, E. (2014). Specificity of the transport of lipid II by FtsW in Escherichia coli. J Biol Chem 289(21): 14707-14718.
  12. den Blaauwen, T. (2013). Prokaryotic cell division: flexible and diverse. Curr Opin Microbiol 16(6): 738-744.
  13. van den Berg van Saparoea, H. B., Glas, M., Vernooij, I. G., Bitter, W., den Blaauwen, T. and Luirink, J. (2013). Fine-mapping the contact sites of the Escherichia coli cell division proteins FtsB and FtsL on the FtsQ protein. J Biol Chem 288(34): 24340-24350.
  14. Verhoeven, G. S., Dogterom, M. and den Blaauwen, T. (2013). Absence of long-range diffusion of OmpA in E. coli is not caused by its peptidoglycan binding domain. BMC Microbiol 13(1): 66.
  15. van der Ploeg, R., Verheul, J., Vischer, N. O., Alexeeva, S., Hoogendoorn, E., Postma, M., Banzhaf, M., Vollmer, W. and den Blaauwen, T. (2013). Colocalization and interaction between elongasome and divisome during a preparative cell division phase in Escherichia coli. Mol Microbiol 87(5): 1074-1087.
  16. van der Ploeg, R. and den Blaauwen, T. (2012). In vivo bacterial morphogenetic protein interactions Chapter 10 Pages 239-262 In Molecular Interactions, Editor: Aurelia Meghea. ISBN 979-953-307-616-3. InTech, Available from:
    http://www.intechopen.com/books/molecular-interactions/in-vivo-bacterial-morphogenetic-protein-interactions
  17. Leisch, N., Verheul, J., Heindl, N. R., Gruber-Vodicka, H. R., Pende, N., den Blaauwen, T. and Bulgheresi, S. (2012). Growth in width and FtsZ ring longitudinal positioning in a gammaproteobacterial symbiont. Curr Biol 22(19): R831-832.
  18. Banzhaf, M., van den Berg van Saparoea, B., Terrak, M., Fraipont, C., Egan, A., Philippe, J., Zapun, A., Breukink, E., Nguyen-Disteche, M., den Blaauwen, T. and Vollmer, W. (2012). Cooperativity of peptidoglycan synthases active in bacterial cell elongation. Mol Microbiol 85(1): 179-194.
  19. Olrichs, N. K., Aarsman, M. E., Verheul, J., Arnusch, C. J., Martin, N. I., Herve, M., Vollmer, W., de Kruijff, B., Breukink, E. and den Blaauwen, T. (2011). A novel in vivo cell-wall labeling approach sheds new light on peptidoglycan synthesis in Escherichia coli. Chembiochem 12(7): 1124-1133.
  20. Kleinschmidt, J. H., Bulieris, P. V., Qu, J., Dogterom, M. and den Blaauwen, T. (2011). Association of neighboring beta-strands of outer membrane protein A in lipid bilayers revealed by site-directed fluorescence quenching. J Mol Biol 407(2): 316-332.
  21. Fraipont, C., Alexeeva, S., Wolf, B., van der Ploeg, R., Schloesser, M., den Blaauwen, T. and Nguyen-Disteche, M. (2011). The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli. Microbiology 157(Pt 1): 251-259.
  22. Schaffner-Barbero, C., Gil-Redondo, R., Ruiz-Avila, L. B., Huecas, S., Lappchen, T., den Blaauwen, T., Diaz, J. F., Morreale, A. and Andreu, J. M. (2010). Insights into nucleotide recognition by cell division protein FtsZ from a mant-GTP competition assay and molecular dynamics. Biochemistry 49(49): 10458-10472.
  23. Soprova, Z., Sauri, A., van Ulsen, P., Tame, J. R., den Blaauwen, T., Jong, W. S. and Luirink, J. (2010). A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation. J Biol Chem 285(49): 38224-38233.
  24. Typas, A., Banzhaf, M., van den Berg van Saparoea, B., Verheul, J., Biboy, J., Nichols, R. J., Zietek, M., Beilharz, K., Kannenberg, K., von Rechenberg, M., Breukink, E., den Blaauwen, T., Gross, C. A. and Vollmer, W. (2010). Regulation of peptidoglycan synthesis by outer-membrane proteins. Cell 143(7): 1097-1109.
  25. Potluri, L., Karczmarek, A., Verheul, J., Piette, A., Wilkin, J. M., Werth, N., Banzhaf, M., Vollmer, W., Young, K. D., Nguyen-Disteche, M. and den Blaauwen, T. (2010). Septal and lateral wall localization of PBP5, the major D,D-carboxypeptidase of Escherichia coli, requires substrate recognition and membrane attachment. Mol Microbiol 77(2): 300-323.
  26. Alexeeva, S., Gadella, T. W., Jr., Verheul, J., Verhoeven, G. S. and den Blaauwen, T. (2010). Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET. Mol Microbiol 77(2): 384-398.
  27. Mohammadi, T., Ploeger, G. E., Verheul, J., Comvalius, A. D., Martos, A., Alfonso, C., van Marle, J., Rivas, G. and den Blaauwen, T. (2009). The GTPase activity of Escherichia coli FtsZ determines the magnitude of the FtsZ polymer bundling by ZapA in vitro. Biochemistry 48(46): 11056-11066.
  28. Kedrov, A., den Blaauwen, T. and Driessen, A. J. (2009). Thermodynamics of the protein translocation. Methods Enzymol 466: 273-291.
  29. Verhoeven, G. S., Alexeeva, S., Dogterom, M. and den Blaauwen, T. (2009). Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4(8): e6739. (http://dx.plos.org/10.1371/journal.pone.0006739).
  30. den Blaauwen, T., de Pedro, M. A., Nguyen-Disteche, M. and Ayala, J. A. (2008). Morphogenesis of rod-shaped sacculi. FEMS Microbiol Rev 32(2): 321-344.
  31. van den Ent, F., Vinkenvleugel, T. M., Ind, A., West, P., Veprintsev, D., Nanninga, N., den Blaauwen, T. and Lowe, J. (2008). Structural and mutational analysis of the cell division protein FtsQ. Mol Microbiol 68(1): 110-123.
  32. Lappchen, T., Pinas, V. A., Hartog, A. F., Koomen, G. J., Schaffner-Barbero, C., Andreu, J. M., Trambaiolo, D., Lowe, J., Juhem, A., Popov, A. V. and den Blaauwen, T. (2008). Probing FtsZ and tubulin with C8-substituted GTP analogs reveals differences in their nucleotide binding sites. Chem Biol 15(2): 189-199.
  33. Scheffers, D. J., Robichon, C., Haan, G. J., den Blaauwen, T., Koningstein, G., van Bloois, E., Beckwith, J. and Luirink, J. (2007). Contribution of the FtsQ transmembrane segment to localization to the cell division site. J Bacteriol 189(20): 7273-7280.
  34. Mohammadi, T., Karczmarek, A., Crouvoisier, M., Bouhss, A., Mengin-Lecreulx, D. and den Blaauwen, T. (2007). The essential peptidoglycan glycosyltransferase MurG forms a complex with proteins involved in lateral envelope growth as well as with proteins involved in cell division in Escherichia coli. Mol Microbiol 65(4): 1106-1121.
  35. Karczmarek, A., Martinez-Arteaga, R., Alexeeva, S., Hansen, F. G., Vicente, M., Nanninga, N. and den Blaauwen, T. (2007). DNA and origin region segregation are not affected by the transition from rod to sphere after inhibition of Escherichia coli MreB by A22. Mol Microbiol 65(1): 51-63.
  36. Norris, V., den Blaauwen, T., Doi, R. H., Harshey, R. M., Janniere, L., Jimenez-Sanchez, A., Jin, D. J., Levin, P. A., Mileykovskaya, E., Minsky, A., Misevic, G., Ripoll, C., Saier, M., Jr., Skarstad, K. and Thellier, M. (2007). Toward a hyperstructure taxonomy. Annu Rev Microbiol 61: 309-329.
  37. Norris, V., den Blaauwen, T., Cabin-Flaman, A., Doi, R. H., Harshey, R., Janniere, L., Jimenez-Sanchez, A., Jin, D. J., Levin, P. A., Mileykovskaya, E., Minsky, A., Saier, M., Jr. and Skarstad, K. (2007). Functional taxonomy of bacterial hyperstructures. Microbiol Mol Biol Rev 71(1): 230-253.
  38. Jong, W. S., ten Hagen-Jongman, C. M., den Blaauwen, T., Slotboom, D. J., Tame, J. R., Wickstrom, D., de Gier, J. W., Otto, B. R. and Luirink, J. (2007). Limited tolerance towards folded elements during secretion of the autotransporter Hbp. Mol Microbiol 63(5): 1524-1536.
  39. den Blaauwen, T., Aarsman, M. E., Wheeler, L. J. and Nanninga, N. (2006). Pre-replication assembly of E. coli replisome components. Mol Microbiol 62(3): 695-708.
  40. Bertsche, U., Kast, T., Wolf, B., Fraipont, C., Aarsman, M. E., Kannenberg, K., von Rechenberg, M., Nguyen-Disteche, M., den Blaauwen, T., Holtje, J. V. and Vollmer, W. (2006). Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli. Mol Microbiol 61(3): 675-690.
  41. Natale, P., den Blaauwen, T., van der Does, C. and Driessen, A. J. (2005). Conformational state of the SecYEG-bound SecA probed by single tryptophan fluorescence spectroscopy. Biochemistry 44(17): 6424-6432.
  42. Aarsman, M. E., Piette, A., Fraipont, C., Vinkenvleugel, T. M., Nguyen-Disteche, M. and den Blaauwen, T. (2005). Maturation of the Escherichia coli divisome occurs in two steps. Mol Microbiol 55(6): 1631-1645.
  43. Lappchen, T., Hartog, A. F., Pinas, V. A., Koomen, G. J. and den Blaauwen, T. (2005). GTP analogue inhibits polymerization and GTPase activity of the bacterial protein FtsZ without affecting its eukaryotic homologue tubulin. Biochemistry 44(21): 7879-7884.
  44. Sijbrandi, R., Den Blaauwen, T., Tame, J. R., Oudega, B., Luirink, J. and Otto, B. R. (2005). Characterization of an iron-regulated alpha-enolase of Bacteroides fragilis. Microbes Infect 7(1): 9-18.
2 Protocols published
Detection of Protein Interactions in the Cytoplasm and Periplasm of Escherichia coli by Förster Resonance Energy Transfer
This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in Escherichia coli by Förster Resonance Energy Transfer (FRET). The described assay allows for the previously impossible in vivo ...
Immunolabeling of Proteins in situ in Escherichia coli K12 Strains
Authors:  Nienke Buddelmeijer, Mirjam Aarsman and Tanneke den Blaauwen, date: 08/05/2013, view: 4547, Q&A: 0
This protocol was developed to label proteins in bacterial cells with antibodies conjugated to a fluorophore for fluorescence microscopy imaging. The procedure is optimized to minimize morphological changes and also to minimize the amount of ...