Michael Riedelberger Center for Medical Biochemistry, Max Perutz Labs Vienna, Campus Vienna Biocenter, Medical University of Vienna
1 protocol

Sabrina Jenull Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Austria
1 protocol

Miha Tome Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia
1 protocol

Fabian Istel Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Austria
4 protocols

Karl Kuchler
  • Professor of Molecular genetics, Medical University of Vienna
Research focus
  • Immunology
  • Karl Kuchler has a long-standing interest in fungal infection biology, molecular genetics, as well as host immune signaling at their intersections with the molecular mechanisms of antifungal drug resistance. His work discovered a novel TLR7-dependent fungal response pathway in dendritic cells, and the group discovered a molecular mechanism underlying the hyper-inflammatory host response upon fungal infections. Finally, the most work uncovered pivotal roles of fungal HDACs/HATs in controlling antifungal drug resistance, stress response, DNA repair as well as virulence.
  • 6 Author merit


Ph.D., Technical University of Graz, 1986


Peer-reviewed journal articles
• Koprulu, A. D., Kastner, R., Wienerroither, S., Lassnig, C., Putz, E. M., Majer, O., Reutterer, B., Sexl, V., Kuchler, K., Muller, M., Decker, T. and Ellmeier, W. (2013). The tyrosine kinase Btk regulates the macrophage response to Listeria monocytogenes infection. PLoS One 8(3): e60476.
• Hnisz, D., Bardet, A. F., Nobile, C. J., Petryshyn, A., Glaser, W., Schock, U., Stark, A. and Kuchler, K. (2012). A histone deacetylase adjusts transcription kinetics at coding sequences during Candida albicans morphogenesis. PLoS Genet 8(12): e1003118.
• Bourgeois, C. and Kuchler, K. (2012). Fungal pathogens-a sweet and sour treat for toll-like receptors. Front Cell Infect Microbiol 2: 142.
• Tscherner, M., Stappler, E., Hnisz, D. and Kuchler, K. (2012). The histone acetyltransferase Hat1 facilitates DNA damage repair and morphogenesis in Candida albicans. Mol Microbiol 86(5): 1197-1214.
• Ryan, O., Shapiro, R. S., Kurat, C. F., Mayhew, D., Baryshnikova, A., Chin, B., Lin, Z. Y., Cox, M. J., Vizeacoumar, F., Cheung, D., Bahr, S., Tsui, K., Tebbji, F., Sellam, A., Istel, F., Schwarzmuller, T., Reynolds, T. B., Kuchler, K., Gifford, D. K., Whiteway, M., Giaever, G., Nislow, C., Costanzo, M., Gingras, A. C., Mitra, R. D., Andrews, B., Fink, G. R., Cowen, L. E. and Boone, C. (2012). Global gene deletion analysis exploring yeast filamentous growth. Science 337(6100): 1353-1356.
• Majer, O., Bourgeois, C., Zwolanek, F., Lassnig, C., Kerjaschki, D., Mack, M., Muller, M. and Kuchler, K. (2012). Type I interferons promote fatal immunopathology by regulating inflammatory monocytes and neutrophils during Candida infections. PLoS Pathog 8(7): e1002811.
• Tierney, L., Kuchler, K., Rizzetto, L. and Cavalieri, D. (2012). Systems biology of host-fungus interactions: turning complexity into simplicity. Curr Opin Microbiol 15(4): 440-446.
• Tierney, L., Linde, J., Muller, S., Brunke, S., Molina, J. C., Hube, B., Schock, U., Guthke, R. and Kuchler, K. (2012). An Interspecies Regulatory Network Inferred from Simultaneous RNA-seq of Candida albicans Invading Innate Immune Cells. Front Microbiol 3: 85
• Zavrel, M., Majer, O., Kuchler, K. and Rupp, S. (2012). Transcription factor Efg1 shows a haploinsufficiency phenotype in modulating the cell wall architecture and immunogenicity of Candida albicans. Eukaryot Cell 11(2): 129-140
• Hnisz, D., Tscherner, M. and Kuchler, K. (2011). Targeting chromatin in fungal pathogens as a novel therapeutic strategy: histone modification gets infectious. Epigenomics 3(2): 129-132.
• Gregori, C., Glaser, W., Frohner, I. E., Reinoso-Martin, C., Rupp, S., Schuller, C. and Kuchler, K. (2011). Efg1 Controls caspofungin-induced cell aggregation of Candida albicans through the adhesin Als1. Eukaryot Cell 10(12): 1694-1704.
• Klein, C., Kuchler, K. and Valachovic, M. (2011). ABC proteins in yeast and fungal pathogens. Essays Biochem 50(1): 101-119.
• Relloso, M., Aragoneses-Fenoll, L., Lasarte, S., Bourgeois, C., Romera, G., Kuchler, K., Corbi, A. L., Munoz-Fernandez, M. A., Nombela, C., Rodriguez-Fernandez, J. L. and Diez-Orejas, R. (2012). Estradiol impairs the Th17 immune response against Candida albicans. J Leukoc Biol 91(1): 159-165.
• Seider, K., Brunke, S., Schild, L., Jablonowski, N., Wilson, D., Majer, O., Barz, D., Haas, A., Kuchler, K., Schaller, M. and Hube, B. (2011). The facultative intracellular pathogen Candida glabrata subverts macrophage cytokine production and phagolysosome maturation. J Immunol 187(6): 3072-3086.
• Lesiak-Markowicz, I., Vogl, G., Schwarzmuller, T., Speth, C., Lass-Florl, C., Dierich, M. P., Kuchler, K. and Wurzner, R. (2011). Candida albicans Hgt1p, a multifunctional evasion molecule: complement inhibitor, CR3 analogue, and human immunodeficiency virus-binding molecule. J Infect Dis 204(5): 802-809.
• Kuchler, K. (2011). The ABC of ABCs: multidrug resistance and genetic diseases. FEBS J 278(18): 3189.
• Borecka, S., Pinjon, E., Sullivan, D. J., Kuchler, K., Blasko, J., Kulkova, N. and Bujdakova, H. (2011). Cdr2p contributes to fluconazole resistance in Candida dubliniensis clinical isolates. Can J Microbiol 57(5): 416-426.
• Hnisz, D., Tscherner, M. and Kuchler, K. (2011). Morphological and molecular genetic analysis of epigenetic switching of the human fungal pathogen Candida albicans. Methods Mol Biol 734: 303-315.
• Bourgeois, C., Majer, O., Frohner, I. E., Lesiak-Markowicz, I., Hildering, K. S., Glaser, W., Stockinger, S., Decker, T., Akira, S., Muller, M. and Kuchler, K. (2011). Conventional dendritic cells mount a type I IFN response against Candida spp. requiring novel phagosomal TLR7-mediated IFN-β signaling. J Immunol 186(5): 3104-3112.
• Kohut, P., Wustner, D., Hronska, L., Kuchler, K., Hapala, I. and Valachovic, M. (2011). The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast: dehydroergosterol fluorescence study. Biochem Biophys Res Commun 404(1): 233-238.
• Frohner, I. E., Gregori, C., Anrather, D., Roitinger, E., Schuller, C., Ammerer, G. and Kuchler, K. (2010). Weak organic acid stress triggers hyperphosphorylation of the yeast zinc-finger transcription factor War1 and dampens stress adaptation. OMICS 14(5): 575-586.
• Landstetter, N., Glaser, W., Gregori, C., Seipelt, J. and Kuchler, K. (2010). Functional genomics of drug-induced ion homeostasis identifies a novel regulatory crosstalk of iron and zinc regulons in yeast. OMICS 14(6): 651-663.
• Bourgeois, C., Majer, O., Frohner, I. E., Tierney, L. and Kuchler, K. (2010). Fungal attacks on mammalian hosts: pathogen elimination requires sensing and tasting. Curr Opin Microbiol 13(4): 401-408.
• Hnisz, D., Majer, O., Frohner, I. E., Komnenovic, V. and Kuchler, K. (2010). The Set3/Hos2 histone deacetylase complex attenuates cAMP/PKA signaling to regulate morphogenesis and virulence of Candida albicans. PLoS Pathog 6(5): e1000889.
• Jacobsen, I. D., Brunke, S., Seider, K., Schwarzmuller, T., Firon, A., d'Enfert, C., Kuchler, K. and Hube, B. (2010). Candida glabrata persistence in mice does not depend on host immunosuppression and is unaffected by fungal amino acid auxotrophy. Infect Immun 78(3): 1066-1077.
• Ernst, R., Kueppers, P., Stindt, J., Kuchler, K. and Schmitt, L. (2010). Multidrug efflux pumps: substrate selection in ATP-binding cassette multidrug efflux pumps--first come, first served? FEBS J 277(3): 540-549.
• Hnisz, D., Schwarzmuller, T. and Kuchler, K. (2009). Transcriptional loops meet chromatin: a dual-layer network controls white-opaque switching in Candida albicans. Mol Microbiol 74(1): 1-15.
• Rockwell, N. C., Wolfger, H., Kuchler, K. and Thorner, J. (2009). ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae. J Membr Biol 229(1): 27-52.
• Bourgeois, C., Majer, O., Frohner, I. and Kuchler, K. (2009). In vitro systems for studying the interaction of fungal pathogens with primary cells from the mammalian innate immune system. Methods Mol Biol 470: 125-139.
• Frohner, I. E., Bourgeois, C., Yatsyk, K., Majer, O. and Kuchler, K. (2009). Candida albicans cell surface superoxide dismutases degrade host-derived reactive oxygen species to escape innate immune surveillance. Mol Microbiol 71(1): 240-252.
• Jungwirth, H., Ring, J., Mayer, T., Schauer, A., Buttner, S., Eisenberg, T., Carmona-Gutierrez, D., Kuchler, K. and Madeo, F. (2008). Loss of peroxisome function triggers necrosis. FEBS Lett 582(19): 2882-2886.
• Gregori, C., Schuller, C., Frohner, I. E., Ammerer, G. and Kuchler, K. (2008). Weak organic acids trigger conformational changes of the yeast transcription factor War1 in vivo to elicit stress adaptation. J Biol Chem 283(37): 25752-25764.
• Roetzer, A., Gregori, C., Jennings, A. M., Quintin, J., Ferrandon, D., Butler, G., Kuchler, K., Ammerer, G. and Schuller, C. (2008). Candida glabrata environmental stress response involves Saccharomyces cerevisiae Msn2/4 orthologous transcription factors. Mol Microbiol 69(3): 603-620.
• Ernst, R., Kueppers, P., Klein, C. M., Schwarzmueller, T., Kuchler, K. and Schmitt, L. (2008). A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5. Proc Natl Acad Sci U S A 105(13): 5069-5074.
6 Protocols published
Analyzing the Quenchable Iron Pool in Murine Macrophages by Flow Cytometry
Authors:  Michael Riedelberger and Karl Kuchler, date: 03/20/2020, view: 2493, Q&A: 0
Tissue-resident macrophages are pivotal for a tightly-regulated iron metabolism at a cellular and systemic level, since subtle iron alterations increase the susceptibility for microbial infections or drive multiple diseases. However, research on ...
Phenotypic Profiling of Candida glabrata in Liquid Media
Authors:  Fabian Istel, Miha Tome, Sabrina Jenull and Karl Kuchler, date: 04/05/2017, view: 8656, Q&A: 0
Here, we describe a method for a large-scale liquid screening approach in C. glabrata. This liquid media method offers several distinct advantages over solid media approaches. This includes growth measurement on a plate reader instead of ...
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