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Coauthors
John Beatty Department of Microbiology and Immunology, University of British Columbia, Canada
5 protocols
Coreviewers
Indranil Malik University of Michigan
1 protocol

Renate Weizbauer Carnegie Institution for Science
40 protocols

Reviewer
Molly M. Leung
  • University of British Columbia
Research focus
  • Microbiology
  • 3 Author merit

Education

Ph.D. in Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, 2010

Current position

Post-doctorate in Chemical and Biomolecular Engineering, University of California Los Angeles, Los Angeles, California, USA

Publications

  1. Leung, M. M., Brimacombe, C. A. and Beatty, J. T. (2013). Transcriptional regulation of the Rhodobacter capsulatus response regulator CtrA. Microbiology 159(Pt 1): 96-106.
  2. Leung, M. M., Brimacombe, C. A., Spiegelman, G. B. and Beatty, J. T. (2012). The GtaR protein negatively regulates transcription of the gtaRI operon and modulates gene transfer agent (RcGTA) expression in Rhodobacter capsulatus. Mol Microbiol 83(4): 759-774.
  3. Leung, M. M., Florizone, S. M., Taylor, T. A., Lang, A. S. and Beatty, J. T. (2010). The gene transfer agent of Rhodobacter capsulatus. Adv Exp Med Biol 675: 253-264.
  4. Tuschak, C., Leung, M. M., Beatty, J. T. and Overmann, J. (2005). The puf operon of the purple sulfur bacterium Amoebobacter purpureus: structure, transcription and phylogenetic analysis. Arch Microbiol 183(6): 431-443.
3 Protocols published
Bacterial Conjugation in Rhodobacter capsulatus
Authors:  Molly M. Leung and John Thomas Beatty, date: 07/05/2013, view: 7182, Q&A: 0
Bacterial conjugation of plasmids is the common method of introducing foreign DNA into Rhodobacter capsulatus because transformational systems have not been shown as efficient methods of introducing DNA to R. capsulatus. For R. ...
Rhodobacter capsulatus Gene Transfer Agent Transduction Assay
Authors:  Molly M. Leung and John Thomas Beatty, date: 02/20/2013, view: 5888, Q&A: 0
The gene transfer agent (GTA) is a bacteriophage-like particle that transfers genomic DNA from a donor to a recipient bacterium. The Rhodobacter capsulatus GTA (RcGTA) was the first to be studied and this protocol has been optimized for ...
1 Protocol reviewed
Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
Authors:  Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp, date: 10/20/2018, view: 14, Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...
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