Isabelle Plo-Azevedo
  • INSERM U1009, Gustave Roussy, France
  • 2 Author merit
2 Protocols published
Protein Translation Study – Label Protein with S35 Methionine in Cells
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 17711, Q&A: 2
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...
Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 9743, Q&A: 0
The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34+ cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34+ ...
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