Salma Merchant —BIO-PROTOCOL

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Isabelle Plo-Azevedo INSERM U1009, Gustave Roussy, France
2 protocols

Agnieszka Pastula Umeå University Umeå
6 protocols

Fanglian He Bio-protocol
205 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
95 protocols

HAILONG ZHANG Neoletix Lab, Inc
1 protocol

Editor

Salma Merchant

Post-Doc, UT Southwestern

Research focus

Stem cell
Hematopoietic Stem cell, Hematopoietic malignancies, Proteostasis, Clonal hematopoiesis, Mitochondria biology

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University Paris XI, 2013

Lab information

Morrison Lab
https://cri.utsw.edu/scientists/sean-morrison-laboratory/

Publications

https://scholar.google.com/citations?user=ERikjhYAAAAJ&hl=en&authuser=1

2 Protocols published

Protein Translation Study – Label Protein with S35 Methionine in Cells

Authors: Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 24310, Q&A: 2

To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...

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Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead

Authors: Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 13626, Q&A: 0

The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34⁺ cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34⁺ ...

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17 Protocols reviewed

AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells

Authors: Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff, date: 04/20/2021, view: 734, Q&A: 0

Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role

...

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Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)

Authors: Himasha M. Perera and Michael A. Trakselis, date: 06/20/2020, view: 1693, Q&A: 0

The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication ...

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Jianan Gong

Walter and Eliza Hall institute