Isabelle Plo-Azevedo INSERM U1009, Gustave Roussy, France
2 protocols
Agnieszka Pastula Umeå University Umeå
6 protocols

Fanglian He Bio-protocol
205 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
95 protocols

HAILONG ZHANG Neoletix Lab, Inc
1 protocol

Editor
Salma Merchant
  • Post-Doc, UT Southwestern
Research focus
  • Stem cell
  • Hematopoietic Stem cell, Hematopoietic malignancies, Proteostasis, Clonal hematopoiesis, Mitochondria biology
  • 2 Author merit
2 Protocols published
Protein Translation Study – Label Protein with S35 Methionine in Cells
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 24310, Q&A: 2
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...
Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead
Authors:  Salma Hasan and Isabelle Plo, date: 11/05/2012, view: 13626, Q&A: 0
The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34+ cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34+ ...
17 Protocols reviewed
AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells
Authors:  Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff, date: 04/20/2021, view: 734, Q&A: 0

Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role

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Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
Authors:  Himasha M. Perera and Michael A. Trakselis, date: 06/20/2020, view: 1693, Q&A: 0
The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication ...
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