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A peer-reviewed protocol journal. No publication fee; no access fee.
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Published
Edited
Reviewed
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Isabelle Plo-Azevedo
INSERM U1009, Gustave Roussy, France
2 protocols
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Gal Haimovich
Molecular Genetics, Weizmann Institute of Science, Israel
81 protocols
Ivan Zanoni
Harvard Medical School
78 protocols
Jyotiska Chaudhuri
Buck Institute for Research on Aging
28 protocols
Kathrin Sutter
University of Duisburg-Essen
14 protocols
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Reviewer
Salma Merchant
Post-Doc, Salma Mercahnt
Research focus
Stem cell
Hematopoietic Stem cell, Hematopoietic malignancies, Proteostasis, Clonal hematopoiesis, Mitochondria biology
Contributions
2
Author merit
Reviewer merit
Editor merit
Education
PhD, University Paris XI, 2013
Lab information
Morrison Lab
Publications
https://scholar.google.com/citations?user=ERikjhYAAAAJ&hl=en&authuser=1
2 Protocols published
Protein Translation Study – Label Protein with S35 Methionine in Cells
Authors:
Salma Hasan
and
Isabelle Plo
,
date:
11/05/2012,
view:
21029,
Q&A:
2
To follow protein synthesis, cells should be incubated with radioactive amino acid such as [35S] methionine during mRNA translation. Then, the neosynthetized protein will be identified by an autoradiography after immunoprecipitation with a specific ...
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Jianan Gong
Walter and Eliza Hall institute
Hi, when you maintain your cells in the methionine free media, have you tested whether cells stop making new proteins? Thanks
3/26/2018 10:31:13 PM
Reply
Isolation of Human Blood Progenitor and Stem Cells from Peripheral Blood by Magnetic Bead
Authors:
Salma Hasan
and
Isabelle Plo
,
date:
11/05/2012,
view:
11738,
Q&A:
0
The antigen CD34 is a well-known marker present on human progenitor and stem cells. This protocol explains the isolation of CD34
+
cells from peripheral blood using magnetic bead separation technique. The approximate abundance of CD34
+
...
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4 Protocols reviewed
3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells
Authors:
Veronica R. Placencio-Hickok
,
Manabu Kato
and
Neil A. Bhowmick
,
date:
05/05/2019,
view:
1321,
Q&A:
0
Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor progression. This was established using transgenic mouse models. Paracrine interactions between fibroblasts and epithelial cells were further interrogated using ...
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Isolation of Microvascular Endothelial Cells
Authors:
Kenneth C.P. Cheung
and
Federica M. Marelli-Berg
,
date:
06/20/2018,
view:
6229,
Q&A:
0
The vascular endothelium is essential to normal vascular homeostasis. Its dysfunction participates in various cardiovascular disorders. Murine endothelial cell culture is an important tool for cardiovascular disease research. This protocol ...
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