Shailesh Kumar National Institute of Health
1 protocol

Reviewer
Salim Gasmi
  • Doctor, Laboratory of toxicology, university of Tebessa.
Research focus
  • Environmental science
  • Toxicology. Neurobiology. Environmental pollution
Classic Labyrinth Test for Neurobehavioral Evaluation in Wistar Rats
Author:  Salim Gasmi, date: 09/20/2018, view: 3151, Q&A: 0
The Classic Labyrinth Test (CLT) is a simple way to evaluate behaviors in rodents such as learning ability, memory, and anxiety. The protocol presented here describes the procedure for use with rats, but the protocol can also be adapted for use in mice if a smaller device is used. In short, the CLT uses a square-shaped maze with a starting point ...
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Cassical labyrinth test for rats
[Feedback 1] The Classic Labyrinth Test (CLT) is a simple way to evaluate behaviors in rodents such as learning ability, memory, and anxiety. The protocol presented here describes the procedure for use with rats, but the protocol can also be adapted for use in mice if a smaller device is used. In short, the CLT uses a square-shaped maze with a starting 

Osteoblast Sorting and Intracellular Staining of CXCL12
Authors:  Weihuan Wang, Gurnoor Majhail, Cui Lui and Lan Zhou, date: 05/20/2018, view: 3722, Q&A: 0
Osteoblasts are bone marrow endosteum-lining niche cells playing important roles in the regulation of hematopoietic stem cells by secreting factors and cell adhesion molecules. Characterization of primary osteoblasts has been achieved through culture of outgrowth of collagenase treated bone. Immunophenotyping and flow-based analysis of long bone ...
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Osteoblast Sorting and Intracellular Staining of CXCL12

Analysis of Myosin II Minifilament Orientation at Epithelial Zonula Adherens
Authors:  Magdalene Michael, Xuan Liang and Guillermo A. Gomez, date: 12/05/2016, view: 5837, Q&A: 0
Non-muscle myosin II (NMII) form bipolar filaments, which bind F-actin to exert cellular contractility during physiological processes (Vicente-Manzanares et al., 2009). Using a combinatorial approach to fluorescently label both N- and C-termini of the NMII heavy chain, recent works have demonstrated the ability to visualize NMII bipolar ...
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Analysis of Myosin II Minifilament Orientation at Epithelial Zonula Adherens

Macrophage Inflammatory Assay
Authors:  Thomas J. Bartosh and Joni H. Ylostalo, date: 07/20/2014, view: 23988, Q&A: 0
Macrophages represent a widely distributed and functionally diverse population of innate myeloid cells involved in inflammatory response to pathogens, tissue homeostasis and tissue repair (Murray and Wynn, 2011). Macrophages can be broadly grouped into two subpopulations with opposing activites: M1 or pro-inflammatory macrophages that promote ...
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Macrophage Inflammatory Assay

Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Authors:  Ran Wang and Sumaira Z. Hasnain, date: 07/20/2017, view: 12544, Q&A: 0
Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 Da. These large proteins are densely substituted with short glycan chains, which have many important ...
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Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology

Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 7863, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is engineered with an N-terminus glutamic acid tag (E-tag or En, where n = the number of glutamic acid in ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Measuring CD38 Hydrolase and Cyclase Activities: 1,N6-Ethenonicotinamide Adenine Dinucleotide (ε-NAD) and Nicotinamide Guanine Dinucleotide (NGD) Fluorescence-based Methods
CD38 is a multifunctional enzyme involved in calcium signaling and Nicotinamide Adenine Dinucleotide (NAD+) metabolism. Through its major activity, the hydrolysis of NAD+, CD38 helps maintain the appropriate levels of this molecule for all NAD+-dependent metabolic processes to occur. Due to current advances and ...
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Measuring CD38 Hydrolase and Cyclase Activities: 1,N6-Ethenonicotinamide Adenine Dinucleotide (ε-NAD) and Nicotinamide Guanine Dinucleotide (NGD) Fluorescence-based Methods

Dye-uptake Experiment through Connexin Hemichannels
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 8860, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are expressed in many cell types, e.g. epithelial cells, astrocytes and immune cells. Hemichannels ...
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Dye-uptake Experiment through Connexin Hemichannels

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