pNP Transgenic RNAi System Manual in Drosophila
[Abstract] Much of our knowledge about the mechanisms underlying biological processes relies on genetic approaches, whereby gene activity is reduced and the phenotypic consequences of perturbation are analyzed in detail. For functional genomic studies, a specific, systematic, and cost-effective manner is critical. Transgenic RNAi system is the top priority ...
Flip-flop Mediated Conditional Gene Inactivation in Drosophila
[Abstract] Mosaic analysis in Drosophila, an important tool to assess cellular phenotypes of mutants in an otherwise heterozygous background, relies on mitosis. Hence, it cannot be used to inactivate gene function in mitotically inactive, terminally differentiated cells such as neurons. To address this issue, we developed “Flip-flop”, a novel, ...
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Authors: Kathrin Marter,
Oliver Kobler,
Ines Erdmann,
Elaheh Soleimanpour,
Peter Landgraf,
Anke Müller,
Julia Abele,
Ulrich Thomas and
Daniela C. Dieterich,
date: 01/20/2019,
view: 1078,
Q&A: 0
[Abstract] Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids. Click chemistry has been widely used to covalently, selectively, and efficiently attach probes such as fluorophores or biotin to small ...
flySAM Transgenic CRISPRa System Manual
[Abstract] Powerful and general methods that can enhance gene expression are useful to systematically study gene function. To date, compared with the methods in generating loss-of-function mutants, methods to achieve gain-of-function are limited. The entire field in Drosophila has relied heavily on the Gal4/UAS:cDNA overexpression system developed ...
CRISPR-Cas9 Mediated Genome Editing in Drosophila
[Abstract] In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in Drosophila. In this optimized system, the ...
Variable Dose Analysis: A Novel High-throughput RNAi Screening Method for Drosophila Cells
[Abstract] Genetic screens are a powerful approach to identify previously uncharacterized genes involved in specific biological processes. Several technologies have been developed for high-throughput screens using reagents such as RNAi or CRISPR, and each approach is associated with specific advantages and disadvantages. Variable Dose Analysis (VDA), is an ...
Testing for Assortative Mating by Diet in Drosophila melanogaster
Authors: Philip T Leftwich and
Tracey Chapman,
date: 10/20/2018,
view: 799,
Q&A: 0
[Abstract] Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe ...
Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations
[Abstract] Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...