Home About Us

Arthropods

Latest Protocol Video

Accelerated Snowmelt Protocol to Simulate Climate Change Induced Impacts on Snowpack Dependent Ecosystems

Categories

Protocols in Current Issue

Phototactic T-maze Behavioral Assay for Comparing the Functionality of Color-sensitive Photoreceptor Subtypes in the Drosophila Visual System

Authors: Hunter S. Shaw, Joe Larkin and Yong Rao, date: 03/20/2020, view: 96, Q&A: 0

The Drosophila retina contains light-sensitive photoreceptors (R cells) with distinct spectral sensitivities that allow them to distinguish light by its spectral composition. R7 and R8 photoreceptors are important for color vision, and can ...

By Date By View

Phototactic T-maze Behavioral Assay for Comparing the Functionality of Color-sensitive Photoreceptor Subtypes in the Drosophila Visual System

Authors: Hunter S. Shaw, Joe Larkin and Yong Rao, date: 03/20/2020, view: 96, Q&A: 0

[Abstract] The Drosophila retina contains light-sensitive photoreceptors (R cells) with distinct spectral sensitivities that allow them to distinguish light by its spectral composition. R7 and R8 photoreceptors are important for color vision, and can be further classified into pale (p) or yellow (y) subtypes depending on the rhodopsin expressed. ...

Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana

Authors: Yanrong You, Chunpeng An and Chuanyou Li, date: 03/05/2020, view: 251, Q&A: 0

[Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...

ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach

Authors: Junaid Akhtar, Piyush More and Steffen Albrecht, date: 02/20/2020, view: 250, Q&A: 0

[Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...

Preparation of Red Palm Weevil Rhynchophorus Ferrugineus (Olivier) (Coleoptera: Dryophthoridae) Germ-free Larvae for Host-gut Microbes Interaction Studies

Authors: Abrar Muhammad, Prosper Habineza, Youming Hou and Zhanghong Shi, date: 12/20/2019, view: 638, Q&A: 0

[Abstract] Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a devastating pest of palm trees worldwide. RPW gut is colonized by diverse bacterial species which profoundly influence host development and nutritional metabolism. However, the molecular mechanisms behind the interactions between RPW and its gut microbiota remain mostly ...

Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue of Drosophila Larval Brains

Authors: Maribel Franco and Ana Carmena, date: 11/20/2019, view: 514, Q&A: 0

[Abstract] The positioning and the cleavage plane orientation of mitotic cells in pseudostratified epithelia (PSE) must be tightly regulated since failures in any of these processes might have fatal consequences during development. Here we present a simple method to determine the spindle orientation as well as the positioning of neuroepithelial mitotic cells ...

Determination of Chromatin Accessibility in Drosophila Midgut Enterocytes by in situ 5mC Labeling

Authors: Eliya Bitman-Lotan, Hector Rincon-Arano, Gal Raz and Amir Orian, date: 11/20/2019, view: 593, Q&A: 0

[Abstract] Regulation of gene expression involves dynamic changes in chromatin organization, where in many cases open chromatin structure correlates with gene activation. Several methods enable monitoring changes in chromatin accessibility, including ATAC-seq, FAIRE-seq, MNase-seq and DNAse-seq methods, which involve Next-generation-sequencing (NGS). ...

Characterization of Biological Motion Using Motion Sensing Superpixels

Authors: Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu, date: 09/20/2019, view: 797, Q&A: 0

[Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay

Authors: Alex Sohr, Lijuan Du and Sougata Roy, date: 08/20/2019, view: 944, Q&A: 0

[Abstract] Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...

Optogenetic Inactivation of Transcription Factors in the Early Embryo of Drosophila

Authors: Stephen L. McDaniel and Melissa M. Harrison, date: 07/05/2019, view: 1413, Q&A: 0

[Abstract] The early embryo of Drosophila melanogaster exists as a rapidly dividing syncytium of nuclei that are transcriptionally silent. Maternally deposited factors are required to awaken the genome and assist in the transition from maternal to zygotic control of development. Because many of these essential factors are maternally deposited and ...

Measurement of Sleep and Arousal in Drosophila

Authors: Margaret E. Driscoll, Callen Hyland and Divya Sitaraman, date: 06/20/2019, view: 1673, Q&A: 0

[Abstract] Sleep is a conserved neurobehavioral state observed in animals with sufficiently complex nervous systems and is critical for survival. While the exact function of sleep remains unknown, the lack of sleep can have a range of physiological and behavioral effects. Studies in invertebrates and vertebrates have identified conserved neural mechanisms ...

Find out more

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.