Protocols in Current Issue
    Testing for Assortative Mating by Diet in Drosophila melanogaster
    Authors:  Philip T Leftwich and Tracey Chapman, date: 10/20/2018, view: 279, Q&A: 0
    [Abstract] Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe ...
    Drosophila Endurance Training and Assessment of Its Effects on Systemic Adaptations
    Authors:  Deena Damschroder , Tyler Cobb, Alyson Sujkowski and Robert Wessells, date: 10/05/2018, view: 357, Q&A: 0
    [Abstract] Exercise induces beneficial systemic adaptations that reduce the incidence of age-related diseases. However, the molecular pathways that elicit these adaptations are not well understood. Understanding the molecular mechanisms that underlie the exercise response can lead to widely beneficial therapies. Large populations, relatively short lifespan, ...
    Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis
    Authors:  Yuping Cai, Nan Liu and Zheng-Jiang Zhu, date: 09/20/2018, view: 489, Q&A: 0
    [Abstract] Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment ...
    Enzymatic Assays and Enzyme Histochemistry of Tuta absoluta Feeding on Tomato Leaves
    Authors:  Rim Hamza, José P. Beltrán and Luis A. Cañas, date: 09/05/2018, view: 512, Q&A: 0
    [Abstract] Enzymes play a key role in insect-plant relationships. For a better understanding of these interactions, we analyzed Tuta absoluta digestive enzymes. Here, we describe a detailed protocol for the detection of trypsin and papain-like enzymes in Tuta absoluta larvae by enzyme histochemistry. This assay uses frozen and unfixed ...
    An Inexpensive and Comprehensive Method to Examine and Quantify Field Insect Community Influenced by Host Plant Olfactory Cues
    Authors:  Rupesh Kariyat, Jesus Chavana and Jasleen Kaur, date: 08/20/2018, view: 600, Q&A: 0
    [Abstract] Insect pollinators, herbivores and their natural enemies use olfactory cues emitted by their host plants to locate them. In insect-plant ecology, understanding the mechanisms underlying these interactions are of critical importance, as this bio-communication has both ecological and agricultural applications. However, the first step in such ...
    Arabidopsis-Green Peach Aphid Interaction: Rearing the Insect, No-choice and Fecundity Assays, and Electrical Penetration Graph Technique to Study Insect Feeding Behavior
    Authors:  Vamsi Nalam, Joe Louis, Monika Patel and Jyoti Shah, date: 08/05/2018, view: 663, Q&A: 0
    [Abstract] Aphids constitute a large group of Hemipterans that use their slender stylets to tap into the sieve elements of plants from which they consume copious amounts of phloem sap, thus depriving the plant of photoassimilates. Some aphids also transmit viral diseases of plants. Myzus persicae Sülzer, commonly known as the green peach aphid ...
    Assessing Experience-dependent Tuning of Song Preference in Fruit Flies (Drosophila melanogaster)
    Authors:  Xiaodong Li, Hiroshi Ishimoto and Azusa Kamikouchi, date: 07/20/2018, view: 733, Q&A: 0
    [Abstract] In songbirds and higher mammals, early auditory experience during childhood is critical to detect and discriminate sound patterns in adulthood. However, the neural and molecular nature of this acquired ability remains elusive. Here, we describe a new behavioral paradigm with Drosophila melanogaster to investigate how the auditory ...
    Ex vivo Whole-cell Recordings in Adult Drosophila Brain
    Authors:  Alexa J. Roemmich, Soleil S. Schutte and Diane K. O’Dowd, date: 07/20/2018, view: 802, Q&A: 0
    [Abstract] Cost-effective and efficient, the fruit fly (Drosophila melanogaster) has been used to make many key discoveries in the field of neuroscience and to model a number of neurological disorders. Great strides in understanding have been made using sophisticated molecular genetic tools and behavioral assays. Functional analysis of neural ...
    Muscle Function Assessment Using a Drosophila Larvae Crawling Assay
    Authors:  Yanina Post and Achim Paululat, date: 07/20/2018, view: 649, Q&A: 0
    [Abstract] Here we describe a simple method to measure larval muscle contraction and locomotion behavior. The method enables the user to acquire data, without the necessity of using expensive equipment (Rotstein et al., 2018). To measure contraction and locomotion behaviour, single larvae are positioned at the center of a humidified Petri dish. ...
    Ex vivo Follicle Rupture and in situ Zymography in Drosophila
    Authors:  Elizabeth M Knapp, Lylah D Deady and Jianjun Sun, date: 05/20/2018, view: 1090, Q&A: 0
    [Abstract] Ovulation, the process of releasing a mature oocyte from the ovary, is crucial for animal reproduction. In order for the process of ovulation to occur, a follicle must be fully matured and signaled to rupture from the ovary. During follicle rupture in both mammals and Drosophila, somatic follicle cells are enzymatically degraded to allow ...

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