Biological Engineering

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    Protocols in Current Issue
    Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)
    Authors:  Simao Coelho, Jongho Baek, J. Justin Gooding and Katharina Gaus, date: 07/05/2021, view: 1547, Q&A: 0
    [Abstract]

    The data quality of high-resolution imaging can be markedly improved with active stabilization, which is based on feedback loops within the microscope that maintain the sample in the same location throughout the experiment. The purpose is to provide a highly accurate focus lock, therefore eliminating drift and improving localization precision.

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    A Detailed Protocol for Preparing Millimeter-sized Supergiant Liposomes that Permit Efficient Eukaryotic Cell-free Translation in the Interior
    Authors:  Hajime Takahashi and Atsushi Ogawa, date: 06/20/2021, view: 756, Q&A: 0
    [Abstract]

    Liposomes have been used as a pseudo cell membrane for encapsulating biomolecules and creating an artificial cell in the interior where biochemical reactions can occur. Among the several methods used to prepare biomolecule-encapsulating liposomes, the spontaneous emulsion transfer method is superior to others in that it allows us to readily

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    Inkjet 3D Printing of Polymers Resistant to Fungal Attachment
    Authors:  Yinfeng He, Cindy Vallieres, Morgan R. Alexander, Ricky D. Wildman and Simon V. Avery, date: 05/05/2021, view: 1862, Q&A: 0
    [Abstract]

    Inkjet 3D printing is an additive manufacturing method that allows the user to produce a small batch of customized devices for comparative study versus commercial products. Here, we describe the use of a commercial 2D ink development system (Dimatix material printing) to manufacture small batches of 3D medical or other devices using a recently

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    Multilayered Fabrication Assembly Technique to Engineer a Corneal Stromal Equivalent
    Authors:  Julia Fernández-Pérez, Peter W. Madden and Mark Ahearne, date: 03/20/2021, view: 1133, Q&A: 0
    [Abstract]

    Tissue engineering has emerged as a strategy to combat the donor shortage of human corneas for transplantation. Synthetic corneal substitutes are currently unable to support the normal phenotype of human cells and so decellularized animal corneas have been deployed to more closely provide the topographical and biochemical cues to promote cell

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