Isolation and Maintenance of Striatal Neurons
Authors: Luana Naia and
A. Cristina Rego,
date: 04/20/2018,
view: 3558,
Q&A: 0
[Abstract] Primary cultures of murine striatal neurons are widely used to explore cellular mechanisms in neurobiology, including brain diseases. Here we describe a detailed and standardized protocol to dissect and culture embryonic murine striatal neurons GABA-positive/DARPP-32-positive for 12 days in vitro, when they show good neuronal cell ...
Olfactory Bulb (OB) Transplants
Authors: Maria Savvaki and
Domna Karagogeos,
date: 09/05/2016,
view: 4767,
Q&A: 0
[Abstract] Transplantation in mouse brain slices is a powerful tool in order to study axon targeting and migrational events during development. Taking advantage of donors and recipients belonging to different genotypes, this technique allows researchers to assess the contribution of donor and/or recipient tissue by performing various combinations and to ...
X-gal Staining on Adult Mouse Brain Sections
Authors: Hiroshi Kokubu and
Janghoo Lim,
date: 03/05/2014,
view: 12858,
Q&A: 0
[Abstract] Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique is regularly used, it requires high quality of specific antibodies and there is no good quality of ...
Optical Clearing Using SeeDB
Authors: Meng-Tsen Ke,
Satoshi Fujimoto and
Takeshi Imai,
date: 02/05/2014,
view: 19544,
Q&A: 1
[Abstract] We describe a water-based optical clearing agent, SeeDB (See Deep Brain), which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. SeeDB is a saturated solution of fructose (80.2% w/w) in water with 0.5% α-thioglycerol. In standard SeeDB optical ...
Dissection of Different Areas from Mouse Hippocampus
Author: Faraz A. Sultan,
date: 11/05/2013,
view: 16523,
Q&A: 0
[Abstract] The hippocampus modulates a number of modules including memory consolidation, spatial navigation, temporal processing and emotion. A banana-shaped structure, the hippocampus is constituted of morphologically distinct subregions including the dentate gyrus, CA3 and CA1 (here, we do not distinguish the “hippocampus proper” which consists only of ...