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    Protocols in Current Issue
    Agrobacterium tumefaciens-mediated Transformation of Walnut (Juglans regia)
    Authors:  Sriema L. Walawage, Charles A. Leslie, Matthew A. Escobar and Abhaya M. Dandekar, date: 10/05/2014, view: 7638, Q&A: 0
    [Abstract] Like many woody plant species, walnut (Juglans regia) can be difficult to genetically transform and regenerate. However, somatic embryos have been used successfully for over two decades as a target tissue for transformation and regeneration of transgenic walnut plants. Walnut somatic embryos, initiated originally from developing zygotic ...
    Assays of Polyphenol Oxidase Activity in Walnut Leaf Tissue
    Authors:  Ross Gertzen and Matthew A. Escobar, date: 08/20/2014, view: 13038, Q&A: 0
    [Abstract] Polyphenol oxidase (PPO) is an enzyme that catalyzes the hydroxylation of monophenols into ortho-diphenols (cresolase activity) and the oxidation of o-diphenols into quinones (catecholase activity) (Figure 1). These quinones spontaneously polymerize to form dark-colored phytomelanins, most often seen in the browning of damaged plant ...



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