Plant Science

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    Protocols in Current Issue
    TUNEL Assay to Assess Extent of DNA Fragmentation and Programmed Cell Death in Root Cells under Various Stress Conditions
    Authors:  Amit K. Tripathi, Ashwani Pareek and Sneh Lata Singla-Pareek, date: 08/20/2017, view: 6729, Q&A: 0
    [Abstract] DNA damage is one of the common consequences of exposure to various stress conditions. Different methods have been developed to accurately assess DNA damage and fragmentation in cells and tissues exposed to different stress agents. However, owing to the presence of firm cellulosic cell wall and phenolics, plant cells and tissues are not easily ...
    A Golden Gate-based Protocol for Assembly of Multiplexed gRNA Expression Arrays for CRISPR/Cas9
    Authors:  Johan Vad-Nielsen, Lin Lin , Kristopher Torp Jensen, Anders Lade Nielsen and Yonglun Luo, date: 12/05/2016, view: 12666, Q&A: 1
    [Abstract] The CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein 9 (Cas9) has become the most broadly used and powerful tool for genome editing. Many applications of CRISPR-Cas9 require the delivery of multiple small guide RNAs (gRNAs) into the same cell in order to achieve multiplexed gene editing or regulation. Using ...
    Detection of the Cell Proliferation Zone in Leaves by Using EdU
    Authors:  Hokuto Nakayama, Kensuke Kawade, Hirokazu Tsukaya and Seisuke Kimura, date: 09/20/2015, view: 5304, Q&A: 0
    [Abstract] Visualization of nuclei in S-phase cells in tissues is important for not only cell cycle research but also developmental research because morphogenesis is usually achieved by a combination of cell proliferation and cell expansion. Recently, DNA labeling with 5-ethynyl-2′-deoxyuridine (EdU), which is an analog of thymidine, has been used to ...
    TUNEL Assay in Kiwifruit Stigmatic Arms
    [Abstract] Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) is a method for detecting DNA fragmentation by labelling the 3' terminal end of nucleic acids. This method can be used both in animal and plant tissues. In animal tissues, the use of Proteinase K is sufficient for permeabilizing the cells and to obtain optimal ...
    DNA Fragmentation Analysis
    Authors:  Kei-ichiro Mishiba, Yukihiro Nagashima, Noriko Hayashi and Nozomu Koizumi, date: 08/05/2014, view: 11332, Q&A: 0
    [Abstract] DNA fragmentation with length corresponding to multiple integer of approximately 180 base pairs is a distinct feature of apoptosis in animals and programmed cell death in plants. This feature can simply be detected by DNA gel electrophoresis followed by ethidium bromide staining, although in some cases it is difficult to distinguish the DNA ...
    Detection of DNA Methylation Changes Surrounding Transposable Elements
    Authors:  Beery Yaakov and Khalil Kashkush, date: 06/05/2013, view: 7604, Q&A: 0
    [Abstract] Transposable elements (TEs) are a major component of all genomes, thus the epigenetic mechanisms controlling their activity is an important field of study. Cytosine methylation is one of the factors regulating the transcription and transposition of TEs, alongside Histone modifications and small RNAs. Adapter PCR-based methods [such as Amplified ...



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