Plant Science

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    Protocols in Current Issue
    Expression, Purification and Crystallization of Recombinant Arabidopsis Monogalactosyldiacylglycerol Synthase (MGD1)
    Authors:  Joana Rocha, Valerie Chazalet and Christelle Breton, date: 12/20/2016, view: 4072, Q&A: 0
    [Abstract] In plant cells, galactolipids are predominant, representing up to 50% of the lipid content in photosynthetic tissues. Galactolipid synthesis is initiated by MGDG synthases (MGDs), which use UDP-galactose as a donor sugar and diacylglycerol (DAG) as acceptor, to form monogalactosyldiacylglycerol (MGDG). This protocol is used to produce a ...
    Heterologous Expression and Purification of Catalytic Domain of CESA1 from Arabidopsis thaliana
    Authors:  Venu Gopal Vandavasi and Hugh O’ Neill, date: 10/20/2016, view: 4047, Q&A: 0
    [Abstract] Heterologous expression of plant cellulose synthase (CESA) and its purification has remained a challenge for decades impeding detailed biophysical, biochemical and structural characterization of this key enzyme. An in-depth knowledge of structure and function of CESA proteins would enable us to better understand the hierarchical structure of the ...
    Localization and Topology of Thylakoid Membrane Proteins in Land Plants
    Authors:  Salar Torabi, Magdalena Plöchinger and Jörg Meurer, date: 12/20/2014, view: 7759, Q&A: 0
    [Abstract] Thylakoids are a formation of flattened membrane vesicles and protein complexes found in cyanobacteria, algae and plants. In the chloroplasts of land plants the thylakoid membrane systems form a network of densely packed stacks called grana lamellae, which are connected by unstacked stroma lamellae. Photosystem II is mainly localized in the ...
    2D Diagonal Redox SDS-PAGE of Proteins
    Authors:  Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 10583, Q&A: 0
    [Abstract] 2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent. ...



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