Cancer Biology


    Protocols in Current Issue
    SMART (Single Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage
    [Abstract] DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, through DNA end-resection, of a long 3′ single-strand DNA stretch, necessary to prime DNA synthesis ...
    Neutral Comet Assay
    Authors:  Elisa Boutet-Robinet, Didier Trouche and Yvan Canitrot, date: 09/20/2013, view: 31918, Q&A: 1
    [Abstract] The Comet assay (or Single Cell Gel Electrophoresis assay) is a sensitive technique to detect DNA damage at the level of an individual cell. This technique is based on micro-electrophoresis of cells DNA content. Briefly, cells are embedded in agarose, lysed and submitted to an electric field, before the staining step with a fluorescent DNA binding ...
    Homologous Recombination Assay
    Authors:  Yvan Canitrot and Didier Trouche, date: 09/20/2013, view: 16126, Q&A: 0
    [Abstract] Repair of double strand break by homologous recombination was examined using U2OS cells or RG37 cells harbouring specific substrate developed by Puget et al. (2005) and Dumay et al. (2006), respectively, to measure the repair of DNA double strand breaks by homologous recombination. The substrate is composed of two inactive copies ...
    End-synapsis Assay
    Authors:  Jessica Cottarel and Patrick Calsou, date: 09/20/2013, view: 7340, Q&A: 0
    [Abstract] Many environmental agents induce double-strand breaks (DSBs) in DNA. Unrepaired or improperly repaired DSBs can lead to cell death or cancer. Nonhomologous end joining is the primary DNA double-strand break repair pathway in eukaryotes. During NHEJ pathway, several proteins recognize and bind DNA ends, bring the ends in a synaptic complex and, ...
    Genomic Signature of Homologous Recombination Deficiency in Breast and Ovarian Cancers
    Authors:  Tatiana Popova, Elodie Manié and Marc-Henri Stern , date: 07/05/2013, view: 13054, Q&A: 0
    [Abstract] Homologous recombination deficiency, mainly resulted from BRCA1 or BRCA2 inactivation (so called BRCAness), is found in breast and ovarian cancers. Detection of actual inactivation of BRCA1/2 in a tumor is important for patients’ treatment and follow-up as it may help predicting response to DNA damaging agents and give ...

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