Isolation of Nuclei in Tagged Cell Types (INTACT), RNA Extraction and Ribosomal RNA Degradation to Prepare Material for RNA-Seq
Authors: Mauricio A. Reynoso,
Germain C. Pauluzzi,
Sean Cabanlit,
Joel Velasco,
Jérémie Bazin,
Roger Deal,
Siobhan Brady,
Neelima Sinha,
Julia Bailey-Serres and
Kaisa Kajala,
date: 04/05/2018,
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[Abstract] Gene expression is dynamically regulated on many levels, including chromatin accessibility and transcription. In order to study these nuclear regulatory events, we describe our method to purify nuclei with Isolation of Nuclei in TAgged Cell Types (INTACT). As nuclear RNA is low in polyadenylated transcripts and conventional pulldown methods would ...
RNA Extraction from RNase-Rich Senescing Leaf Samples
Author: Susheng Gan,
date: 08/20/2012,
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[Abstract] Isolation of intact, full-length high quality RNAs is essential for RNA sequencing, reverse transcription PCR analysis of gene expression as well as RNA gel blot analysis. This simple yet easy protocol is developed to meet this need; in addition to regular samples, this protocol is especially good for isolating RNAs from RNase-rich samples such ...