Stem Cell

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    Protocols in Current Issue
    Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier
    [Abstract] The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice ...
    Implantation of Human Peripheral Corneal Spheres into Cadaveric Human Corneal Tissue
    Authors:  Jeremy John Mathan, Salim Ismail, Jennifer Jane McGhee and Trevor Sherwin, date: 07/20/2017, view: 4525, Q&A: 0
    [Abstract] Stem and progenitor cells isolated from human limbal tissue can be cultured in vitro as spheres. These spheres have potential for use as transplantable elements for the repopulation of corneal tissue (Mathan et al., 2016). Herein we describe the detailed protocol for the implantation of human corneal spheres into cadaveric human ...
    Experimental Liver Fibrosis and Intrasplenic Transplantation of CD45+ Bone Marrow Cells
    Authors:  Prakash Baligar, Sebanta Pokhrel and Asok Mukhopadhyay, date: 10/20/2016, view: 8430, Q&A: 1
    [Abstract] Liver fibrosis results from the excessive collagen deposition (collagen scar) by activated hepatic stellate cells (HpSCs), leading to the inhibition of normal liver regeneration and function. Fibrogenesis is a complex mechanism involving both the synthesis and degradation of matrix proteins by different cell types, mainly macrophages in the liver. ...



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