Neuroscience

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    Protocols in Current Issue
    Whole-mount Immunohistochemistry of Adult Zebrafish Retina for Advanced Imaging
    Authors:  Mikiko Nagashima and Peter F. Hitchcock, date: 12/20/2020, view: 1501, Q&A: 0
    [Abstract]

    Immunohistochemistry is a widely used technique to examine the expression and subcellular localization of proteins. This technique relies on the specificity of antibodies and requires adequate penetration of antibodies into tissues. The latter is especially challenging for thick specimens, such as embryos and other whole-mount preparations. Here

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    Gallyas Silver Impregnation of Myelinated Nerve Fibers
    Authors:  Sabitha Joseph, Hauke B. Werner and Judith Stegmüller, date: 11/20/2019, view: 2542, Q&A: 0
    [Abstract] In the nervous system of vertebrates, nerve impulse propagation is accelerated by the ensheathment of neuronal axons with myelin. Myelin sheaths are molecularly specialized, lipid-rich plasma membrane extensions of Schwann cells in the peripheral nervous system and oligodendrocytes in the central nervous system (CNS). To visualize myelinated nerve ...
    Fluoro-Jade B Staining for Neuronal Cell Death
    Authors:  Nathalie Laflamme, Paul Préfontaine and Serge Rivest, date: 01/05/2016, view: 19259, Q&A: 2
    [Abstract] Fluoro-Jade is a fluorescent derivative used for histological staining of degenerating neurons. This technique is simple and sensitive enough to label distal dendrites, axons, axon terminals as well as neuronal bodies. Fluoro-Jade has excitation and emission peak of 480 and 525 nanometer respectively. It can be visualized using a fluorescein/FITC ...



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