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Confocal Microscopy of Reovirus Transport in Living Dorsal Root Ganglion Neurons

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Expression and Purification of Arabidopsis Transmembrane Protein BCM1 in Saccharomyces cerevisiae

Authors: Peng Wang and Bernhard Grimm, date: 09/20/2020, view: 608, Q&A: 0

[Abstract] Heterologous expression and purification of transmembrane proteins have remained a challenge for decades hampering detailed biochemical and structural characterization of key enzymes and their interacting regulators in multiple metabolic pathways. An in-depth study on the newly identified Arabidopsis thaliana integral membrane protein ...

Determination of Ureides Content in Plant Tissues

Author: Ignacio Lescano, date: 06/05/2020, view: 1080, Q&A: 0

[Abstract] The ureides allantoin and allantoate are the main organic nitrogen compounds transported in several legumes, predominantly from N₂ fixation. Moreover, recent studies point out a remarkable role for allantoin during several stress responses of plants other than legumes. The goal of this protocol is to determine ureides concentration in ...

Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay

Authors: Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida, date: 01/05/2020, view: 1785, Q&A: 0

[Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca²⁺-dependent and redox-dependent signals. Conventional in ...

Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings

Authors: Sandeep B. Adavi, Lekshmy Sathee, Birendra K. Padhan, Ompal Singh, Hari S. Meena, Kumar Durgesh and Shailendra K. Jha, date: 10/20/2019, view: 2086, Q&A: 0

[Abstract] Nitric oxide (NO), is a redox-active, endogenous signalling molecule involved in the regulation of numerous processes. It plays a crucial role in adaptation and tolerance to various abiotic and biotic stresses. In higher plants, NO is produced either by enzymatic or non-enzymatic reduction of nitrite and an oxidative pathway requiring a putative ...

An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil

Authors: Alex Williams, Jurriaan Ton and Pierre Pétriacq, date: 05/20/2019, view: 3051, Q&A: 0

[Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...

Examining Autophagy in Plant by Transmission Electron Microscopy (TEM)

Authors: Xiyin Zheng, Chenguang Zhao and Yule Liu, date: 10/20/2018, view: 3123, Q&A: 0

[Abstract] In plants, macroautophagy, here referred as autophagy, is a degradation pathway during which the double-membrane structure named autophagosome engulfs the cargo and then fuses with vacuole for material recycling.

To investigate the process of autophagy, transmission electron microscopy (TEM) was used to monitor the ultrastructure of ...

Separation of Plant 6-Phosphogluconate Dehydrogenase (6PGDH) Isoforms by Non-denaturing Gel Electrophoresis

Authors: Francisco J Corpas, Larisse de Freitas-Silva, Nuria García-Carbonero, Alba Contreras, Fátima Terán, Carmelo Ruíz-Torres and José M. Palma, date: 07/20/2017, view: 5757, Q&A: 1

[Abstract] 6-Phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44) catalyzes the third and irreversible reaction of the pentose phosphate pathway (PPP). It carries out the oxidative decarboxylation of the 6-phosphogluconate to yield ribulose-5-phosphate, carbon dioxide and NADPH. In higher plants, 6PGDH has several subcellular localizations including cytosol, ...

Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of Capparis decidua

Authors: Yvonne Forster, Abdul Ghaffar and Stefan Bienz, date: 02/20/2017, view: 5571, Q&A: 0

[Abstract] Various parts of the caper tree Capparis decidua have found application in traditional medicine. The isolation and structural elucidation of the codonocarpine type alkaloids contained in the root bark, however, is not trivial and has probably led to misinterpretation in the past. This protocol describes the extraction and chromatographic ...

Extraction of Apoplastic Wash Fluids and Leaf Petiole Exudates from Leaves of Arabidopsis thaliana

Authors: Takao Araya, Anne Bohner and Nicolaus von Wirén, date: 12/20/2015, view: 10470, Q&A: 0

[Abstract] The long-distance translocation of metabolites and mineral elements is crucial for plant growth and reproduction. In most cases, source-to-sink translocation of metabolites and minerals requires their passage through the apoplast, irrespective whether they are transported via the xylem or the phloem. This apoplast-mediated pathway is of particular ...

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