Cancer Biology

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    Protocols in Current Issue
    Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis
    [Abstract] Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails to detect localized responses in the lymph ...
    Isolation of Mouse Tumor-Infiltrating Leukocytes by Percoll Gradient Centrifugation
    Authors:  Ying Liu, Keqiang Chen, Chunyan Wang, Wanghua Gong, Teizo Yoshimura, Ji Ming Wang and Mingyong Liu, date: 09/05/2013, view: 37726, Q&A: 1
    [Abstract] A hallmark of cancer-associated inflammation is the infiltration of leukocytes into tumors, which is believed to be recruited by chemokines. Some infiltrating leukocytes such as macrophages often promote tumor growth by producing growth-inducing and angiogenic factors. Here, we described a method of isolating tumor-infiltrating leukocytes with ...



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