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Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels

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Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva

Authors: Raffaella Klima, Giulia Romano, Monsurat Gbadamosi, Aram Megighian and Fabian Feiguin, date: 02/05/2021, view: 186, Q&A: 0

[Abstract]

Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through

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Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex

Authors: Ariel Diaz, Enrique Torre and Manuel Yepes, date: 01/20/2021, view: 534, Q&A: 1

[Abstract]

The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

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Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine

Authors: Muhammad Nauman Arshad and Janice R Naegele, date: 02/20/2020, view: 2233, Q&A: 0

[Abstract] In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice show behavioral seizures within an hour after pilocarpine is injected. With the behavioral scoring ...

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Authors: Victoria Riccio, Miluska Vissa, Angus McQuibban and Peter Kijun Kim, date: 12/20/2019, view: 2656, Q&A: 0

[Abstract] The process of autophagy is an essential cellular mechanism, required to maintain general cell health through the removal of dysfunctional organelles, such as the ER, peroxisomes and mitochondria, as well as protein aggregates, and bacteria. Autophagy is an extremely dynamic process, and tools are constantly being developed to study the various ...

Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

Authors: Panayiotis Tsokas, Bruno Rivard, Changchi Hsieh, James E. Cottrell, André Antonio Fenton and Todd Charlton Sacktor, date: 10/05/2019, view: 1863, Q&A: 0

[Abstract] The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in vivo plasticity. Here we describe a novel method to rapidly suppress gene expression by ...

Estimation of the Readily Releasable Synaptic Vesicle Pool at the Drosophila Larval Neuromuscular Junction

Authors: Pragya Goel, Xiling Li and Dion Dickman, date: 01/05/2019, view: 4327, Q&A: 0

[Abstract] Presynaptic boutons at nerve terminals are densely packed with synaptic vesicles, specialized organelles for rapid and regulated neurotransmitter secretion. Upon depolarization of the nerve terminal, synaptic vesicles fuse at specializations called active zones that are localized at discrete compartments in the plasma membrane to initiate synaptic ...

Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices

Authors: Madelyn I. Mauterer, Paige M. Estave, Katherine M. Holleran and Sara R. Jones, date: 10/05/2018, view: 6361, Q&A: 1

[Abstract] Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that allows sub-second detection of oxidizable chemical species, including monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. This technique has been used to record the physiological dynamics of these neurotransmitters in brain tissue, including their ...

Quantitative Electron Microscopic Assay Using Random Sampling from Single Sections to Test Plastic Synaptic Changes in Hippocampus

Authors: G. Mark Marcello, Lilla E. Szabó, Peter Sotonyi and Bence Rácz, date: 08/05/2018, view: 3445, Q&A: 0

[Abstract] Studies over several decades on the organization of the CA1 hippocampus–a particularly favorable model for learning, memory and certain forms of cognition–have shown that the synaptic network in this brain region is plastic (Fortin et al., 2012). Recent evidence suggests that a number of environmental and endogenous stimuli may have a ...

Obtaining Acute Brain Slices

Authors: Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 15121, Q&A: 1

[Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...

D-serine Measurements in Brain Slices or Other Tissue Explants

Authors: Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 4928, Q&A: 0

[Abstract] D-serine is an atypical amino acid present in the mammalian body (most amino acids in the mammalian body are L-isomers) that is mostly known in neuroscience for its role as a co-agonist controlling the N-methyl D-aspartate receptor (NMDAR). D-serine levels are decreased in patients with schizophrenia and this is thought to mediate, at least in ...

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