Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells
[Abstract] The process of autophagy is an essential cellular mechanism, required to maintain general cell health through the removal of dysfunctional organelles, such as the ER, peroxisomes and mitochondria, as well as protein aggregates, and bacteria. Autophagy is an extremely dynamic process, and tools are constantly being developed to study the various ...
Measurement of Dopamine Using Fast Scan Cyclic Voltammetry in Rodent Brain Slices
[Abstract] Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that allows sub-second detection of oxidizable chemical species, including monoamine neurotransmitters such as dopamine, norepinephrine, and serotonin. This technique has been used to record the physiological dynamics of these neurotransmitters in brain tissue, including their ...
Obtaining Acute Brain Slices
Authors: Thomas Papouin and
Philip G. Haydon,
date: 01/20/2018,
view: 10714,
Q&A: 1
[Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
D-serine Measurements in Brain Slices or Other Tissue Explants
Authors: Thomas Papouin and
Philip G. Haydon,
date: 01/20/2018,
view: 4269,
Q&A: 0
[Abstract] D-serine is an atypical amino acid present in the mammalian body (most amino acids in the mammalian body are L-isomers) that is mostly known in neuroscience for its role as a co-agonist controlling the N-methyl D-aspartate receptor (NMDAR). D-serine levels are decreased in patients with schizophrenia and this is thought to mediate, at least in ...