Cell Biology

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    Protocols in Current Issue
    Assessing Mitochondrial Transport via Cytoplasmic Nanotubular Bridges in Cells
    Authors:  Shravani Mukherjee, Tanveer Ahmad and Anurag Agrawal, date: 08/05/2015, view: 6942, Q&A: 0
    [Abstract] This protocol aims to study intercellular transport of mitochondria, dynamic cellular organelles via tunnelling nanotubes (TNT), a cell membrane extension of cytoskeletal elements. The nanotubular bridges or the tunnelling nanotube highways are one of the emerging new cell-to-cell communication systems which mediates exchange of cellular ...
    Lysosomal Stability Assay
    Authors:  Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä, date: 06/20/2014, view: 14980, Q&A: 0
    [Abstract] This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

    AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the cell it enters the acidic lysosomal compartment where it is protonated and sequestered, shifting its ...



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