Microbiology

Categories

    Protocols in Current Issue
    Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria
    Authors:  Bo Ma, Chao Fang, Jing Zhang, Mingzhi Wang, Xiaoxing Luo and Zheng Hou, date: 03/05/2020, view: 1271, Q&A: 0
    [Abstract] The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to ...
    Rapid Detection of Proliferative Bacteria by Electrical Stimulation
    Authors:  Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally, date: 02/05/2020, view: 2131, Q&A: 0
    [Abstract] Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...
    Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
    Authors:  Michaela Wenzel, Norbert O. E. Vischer, Henrik Strahl and Leendert W. Hamoen, date: 10/20/2018, view: 4762, Q&A: 0
    [Abstract] Membrane fluidity is a key parameter of bacterial membranes that undergoes quick adaptation in response to environmental challenges and has recently emerged as an important factor in the antibacterial mechanism of membrane-targeting antibiotics. The specific level of membrane fluidity is not uniform across the bacterial cell membrane. Rather, ...
    Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
    Authors:  Pengyuan Xiu, Rui Liu, Dechao Zhang and Chaomin Sun, date: 01/05/2018, view: 5085, Q&A: 0
    [Abstract] Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; ...
    Antibiotic Disc Assay for Synechocystis sp. PCC6803
    Authors:  Otilia Cheregi and Christiane Funk, date: 12/20/2016, view: 7174, Q&A: 0
    [Abstract] This protocol describes how to investigate the integrity of the outer cell wall in the cyanobacterium Synechocystis sp. PCC6803 using antibiotics. It is adapted to the agar diffusion test (Bauer et al., 1966), in which filter paper discs impregnated with specified concentrations of antibiotics were placed on agar plates ...
    Single Cell Flow Cytometry Assay for Peptide Uptake by Bacteria
    [Abstract] Antimicrobial peptides (AMPs) can target the bacterial envelope or alternatively have intracellular targets. The latter requires uptake of the peptide by the bacterial cells. The bacterial internalization of an AMP can be evaluated by a fluorescence-based method that couples the use of the fluorescently labelled AMP to the fluorescence quencher ...
    Testing the Effect of UV Radiation on the Survival of Burkholderia glumae
    Authors:  Hari Sharan Karki and Jong Hyun Ham, date: 03/05/2016, view: 7403, Q&A: 0
    [Abstract] Burkholderia glumae (B. glumae) is becoming a serious threat in the major rice producing areas of the world. It was reported that Burkholderia spp., including B. glumae, are adapted to a wide range of ecological niches. Different bacterial strains show different levels of UV tolerance which may be due to the ...
    In vitro Dynamic Model of a Catheterized Bladder and Biofilm Assay
    Authors:  Mario Maierl, Michael Jörger, Patrik Rosker and Andreas Reisner, date: 01/20/2015, view: 9436, Q&A: 0
    [Abstract] Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI) which represent the most frequent nosocomial infections. Understanding of factors relevant for CAUTI pathogenesis and evaluation of new therapeutics or interference strategies requires a model system that mirrors the ...
    Induction of Tigecycline Resistance in Acinetobacter baumannii
    Authors:  Ming-Feng Lin, Yun-You Lin and Chung-Yu Lan, date: 12/05/2014, view: 6575, Q&A: 0
    [Abstract] Multidrug resistance Acinetobacter baumannii (A. baumannii) (MDRAB) has emerged as a serious threat in hospitals in recent years. Currently, there are few antibiotics, including tigecycline, available to treat infections caused by MDRAB effectively. Both tigecycline-resistant and tigecycline-susceptible isogenic strains of MDRAB ...
    Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
    Authors:  Ming-Feng Lin, Yun-You Lin and Chung-Yu Lan, date: 12/05/2014, view: 39429, Q&A: 3
    [Abstract] Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.