Immunology

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    Protocols in Current Issue
    Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
    Authors:  Mélanie Bousquenaud, Pierre-Yves Marie and Yvan Devaux, date: 11/20/2015, view: 4680, Q&A: 0
    [Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution ...
    Rat Aortic Ring Model to Assay Angiogenesis ex vivo
    Authors:  Isabelle Ernens, Bénédicte Lenoir, Yvan Devaux and Daniel R. Wagner, date: 10/20/2015, view: 5815, Q&A: 0
    [Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of ...
    Gastric Aspiration Models
    Authors:  Bruce A. Davidson and Ravi Alluri, date: 11/20/2013, view: 13434, Q&A: 0
    [Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
    1) Normal saline (NS) as the injury vehicle control ...
    Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
    [Abstract] Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan et al., 2012; Oprica et al., 2003). The microdialysis technique allows the local in vivo perfusion of KA and the simultaneous collection of inflammatory mediators, ...



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