Protocols in Current Issue
    Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction

    Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans.

    Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats
    Authors:  Thaisa Meira Sandini, Quentin Greba, Brittney Rose Lins and John George Howland, date: 11/20/2020, view: 1074, Q&A: 0

    Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the

    Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats
    [Abstract] Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we ...
    Permanent Occlusion of the Left Anterior Coronary Artery in the Rat
    Authors:  Mélanie Bousquenaud, Pierre-Yves Marie and Yvan Devaux, date: 11/20/2015, view: 6956, Q&A: 0
    [Abstract] Left ventricular (LV) remodeling occurs in many patients after myocardial infarction (MI). LV remodeling is characterized by progressive ventricular dilatation and contractile dysfunction, consequently to cardiomyocyte hypertrophy and fibrosis. Despite reperfusion therapies, this pathophysiological process is the main cause of cardiac evolution ...
    Rat Aortic Ring Model to Assay Angiogenesis ex vivo
    Authors:  Isabelle Ernens, Bénédicte Lenoir, Yvan Devaux and Daniel R. Wagner, date: 10/20/2015, view: 8659, Q&A: 0
    [Abstract] Angiogenesis is a multifactorial event which requires the migration, proliferation, differentiation and structure rearrangement of endothelial cells. This angiogenic process has been commonly studied using in vitro assays such as Boyden chamber assay, wound healing assay and tube formation assay. These assays mainly use monolayers of ...
    Gastric Aspiration Models
    Authors:  Bruce A. Davidson and Ravi Alluri, date: 11/20/2013, view: 16911, Q&A: 0
    [Abstract] The procedures described below are for producing gastric aspiration pneumonitis in mice with alterations for rats and rabbits described parenthetically. We use 4 different injury vehicles delivered intratracheally to investigate the inflammatory responses to gastric aspiration:
    1) Normal saline (NS) as the injury vehicle control ...
    Evaluation of Caspase-1 Activation and IL-1β Production in A Kainic Acid Microdyalisis Brain Injury Model
    [Abstract] Intracerebral infusion of kainic acid (KA) by a microdialysis probe induces a focal swelling in the brain-perfused area which promotes inflammation (Compan et al., 2012; Oprica et al., 2003). The microdialysis technique allows the local in vivo perfusion of KA and the simultaneous collection of inflammatory mediators, ...

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