Cell Biology

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    Protocols in Current Issue
    Detection of Reactive Oxygen Species Using MitoSOX and CellROX in Zebrafish
    Authors:  Alberto Rissone and Fabio Candotti, date: 10/05/2016, view: 12813, Q&A: 1
    [Abstract] The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the ...
    Histochemical Detection of Superoxide and H2O2 Accumulation in Brassica juncea Seedlings
    Authors:  Deepak Kumar, Mohd Aslam Yusuf, Preeti Singh, Meryam Sardar and Neera Bhalla Sarin, date: 04/20/2014, view: 16736, Q&A: 2
    [Abstract] Plant cells continually produce reactive oxygen species (ROS) as a by-product of aerobic metabolism. Increased production of ROS occurs under unfavorable conditions imposed by various abiotic and biotic factors. Accumulation of ROS is damaging to various cellular components and macromolecules including plasma membrane, nucleic acids, and proteins ...
    Detection of Intracellular Reactive Oxygen Species (CM-H2DCFDA)
    Authors:  Rabii Ameziane-El-Hassani and Corinne Dupuy, date: 01/05/2013, view: 64481, Q&A: 3
    [Abstract] Reactive oxygen species (ROS) play a critical role in cellular physiopathology. ROS are implicated in cell proliferation, signaling pathways, oxidative defense mechanisms responsible for killing of bacteria, thyroid hormonosynthesis, etc. The cellular Redox homeostasis is balanced by oxidants and antioxidants systems. In several diseases (cancer, ...
    DAB Staining and Visualization of Hydrogen Peroxide in Wheat Leaves
    Authors:  Zhaohui Liu and Tim Friesen , date: 12/20/2012, view: 12424, Q&A: 0
    [Abstract] The production of hydrogen peroxide (H2O2) has been recognized as an important feature of plant cells that undergo programmed cell death (PCD) during host-pathogen interaction. Thordal-Christensen et al. (1997) first described a method using chemical 3,3-diaminobenzidine (DAB) to detect the presence and distribution ...



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