Cell Biology

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    Protocols in Current Issue
    Measurement of TLR4 and CD14 Receptor Endocytosis Using Flow Cytometry
    Authors:  Michael S. Schappe and Bimal N. Desai, date: 07/20/2018, view: 5148, Q&A: 0
    [Abstract] After recognizing extracellular bacterial lipopolysaccharide (LPS), the toll-like receptor 4 (TLR4)-CD14 signaling complex initiates two distinct signaling pathways–one from the plasma membrane and the other from the signaling endosomes (Kagan et al., 2008). Understanding the early stages of TLR4 signal transduction therefore requires a ...
    DQ-Red BSA Trafficking Assay in Cultured Cells to Assess Cargo Delivery to Lysosomes
    Authors:  Rituraj Marwaha and Mahak Sharma, date: 10/05/2017, view: 9441, Q&A: 0
    [Abstract] Lysosomes are the terminal end of the endocytic pathway having acidic environment required for active hydrolases that degrade the cargo delivered to these compartments. This process of cargo delivery and degradation by endo-lysosomes is a tightly regulated process and important for maintaining cellular homeostasis. Cargos like EGF (Epidermal ...
    Influenza Virus-cell Fusion Inhibition Assay
    Authors:  Mayo Yasugi and Kazuyoshi Ikuta, date: 12/20/2013, view: 8132, Q&A: 0
    [Abstract] During viral infection to host cells, several viruses undergo the process of endocytosis and pH-dependent fusion. By fusion of viral membrane with host cellular membrane, the viral core invades to host cytoplasm. A part of monoclonal antibodies against viral membrane protein have potential to inhibit the viral fusion step. Here we describe in ...
    Assessment of Human Dendritic Cell Antigen Uptake by Flow Cytometry
    Authors:  Ana Luque, Sonia Cárdenas-Brito, Rut Olivar and Josep M. Aran, date: 11/20/2013, view: 12911, Q&A: 0
    [Abstract] Antigen uptake by dendritic cells is the first key step towards induction of antigen-specific T-cell responses. This flow cytometry-based protocol describes the analysis of dendritic cell uptake of soluble antigens through two different mechanisms: non-specific macropinocytosis (using Lucifer Yelloy CH), and receptor-mediated endocytosis (using DQ ...
    Epidermal Growth Factor (EGF) Receptor Endocytosis Assay in A549 Cells
    Authors:  Sabrina Rizzolio and Luca Tamagnone, date: 08/05/2013, view: 14525, Q&A: 0
    [Abstract] The following endocytosis assay has been optimized to assess EGF-stimulated EGFR endocytosis; but could be modified to assess other ligand-stimulated endocytosis of plasma membrane receptors (for which fluorochrome-conjugated ligands are available to track their receptor internalization). In brief, cells are treated with fluorescent EGF at 4 °C to ...



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