Cell Biology

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    Protocols in Current Issue
    Membrane Flotation Assay
    Authors:  Dorothee A Vogt and Melanie Ott, date: 04/05/2015, view: 8975, Q&A: 0
    [Abstract] Many postitive-stranded RNA viruses, such as Hepatitis C virus (HCV), highjack cellular membranes, including the Golgi, ER, mitchondria, lipid droplets, and utilize them for replication of their RNA genome or assembly of new virions. By investigating how viral proteins associate with cellular membranes we will better understand the roles of ...
    Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
    [Abstract] It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed ...
    Small-scale Subcellular Fractionation with Sucrose Step Gradient
    Authors:  Yuzuru Taguchi and Hermann M. Schätzl, date: 06/05/2014, view: 11105, Q&A: 0
    [Abstract] Here, we introduce the protocol for small-scale and simple subcellular fractionation used in our recent publication (Taguchi et al., 2013), which uses homogenization by passing through needles and sucrose step-gradient.

    Subcellular fractionation is a very useful technique but usually a large number of cells are required. ...
    Cell Fractionation of Pseudomonas aeruginosa
    Authors:  Esteban Paredes-Osses and Kim R. Hardie, date: 10/05/2013, view: 8189, Q&A: 0
    [Abstract] Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein purification strategy (Williams et al., 1998). This protocol has been designed to obtain the ...
    Preparation of Pre- and Post-synaptic Density Fraction from Mouse Cortex
    Authors:  Chengyong Shen and Yong-Jun Chen, date: 09/05/2013, view: 9041, Q&A: 1
    [Abstract] The understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. Here we described an efficient way to isolate the crude synaptosome, presynaptic ...
    Isolation of Growth Cones from Mouse Brain
    Authors:  Iryna Leshchyns’ka and Vladimir Sytnyk, date: 08/05/2013, view: 7586, Q&A: 0
    [Abstract] Growth cones are motile structures at the tips of growing neurites, which play an essential role in regulation of growth and navigation of growing axons and dendrites of neurons in the developing nervous system. This protocol describes isolation of growth cones from the brain tissue from young mice. Growth cones isolated using this protocol have ...
    Subcellular Fractionation of Mouse Brain Homogenates
    Authors:  Ditte Olsen and Camilla Gustafsen, date: 08/05/2013, view: 9557, Q&A: 0
    [Abstract] This subcellular fractionation protocol is used for separation of cellular organelles based on their density. We have designed and optimized the protocol for separation of subcellular compartments of brain homogenates with focus on the localization and trafficking of transmembrane proteins, but we have also successfully used this protocol for ...
    Subcellular Fractionation of Cultured Human Cell Lines
    Authors:  Zhuoyou Yu, Zhiguang Huang and Maria Li Lung, date: 05/05/2013, view: 34947, Q&A: 5
    [Abstract] Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer. Immuno-fluorescent staining and subcellular fractionation can be used to determine localization of a protein. Here we discuss a protocol to separate the ...



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