Neuroscience

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    Protocols in Current Issue
    Retinal Explant Culture
    Authors:  Christine Jolicoeur and Michel Cayouette, date: 01/20/2014, view: 16545, Q&A: 3
    [Abstract] A particularly powerful culture method for the retina is the explant assay, which consists in culturing a small piece of retina on an organotypic filter. Retinal explants can be prepared any time between embryonic day 13 (E13) and postnatal day 4 (P4). Although retinal ganglion cells tend to degenerate shortly after they are generated in explants, ...
    Dissociated Retinal Cell Culture
    Authors:  Christine Jolicoeur and Michel Cayouette, date: 01/20/2014, view: 11651, Q&A: 0
    [Abstract] The retina is a relatively simple and accessible part of the central nervous system, making it a powerful model to study cell fate specification mechanisms. Multipotent retinal progenitor cells (RPCs) give rise to seven major classes of retinal cell types. Mechanisms regulating cell fate choice in the retina depend on both cell intrinsic and ...



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