Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney
Authors: Ming-Zhi Zhang and
Raymond C. Harris,
date: 09/20/2019,
view: 911,
Q&A: 0
[Abstract] Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney is of particular importance. The current protocol describes CD8α immunostaining using a Vectastain ...
Immunohistochemical Identification of Human Skeletal Muscle Macrophages
Authors: Kate Kosmac,
Bailey D. Peck,
R. Grace Walton,
Jyothi Mula,
Philip A. Kern,
Marcas M. Bamman,
Richard A. Dennis,
Cale A. Jacobs,
Christian Lattermann,
Darren L. Johnson and
Charlotte A. Peterson,
date: 06/20/2018,
view: 7872,
Q&A: 0
[Abstract] Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage ...
Quantification of Rae-1 Staining Intensity in Glomeruli
Authors: Roberto Spada and
Domingo F. Barber,
date: 03/05/2016,
view: 4887,
Q&A: 0
[Abstract] NKG2D is expressed on all NK cells and on subsets of NKT, CD8, CD4 and γδ T cells. NKG2D is activated by NKG2D ligands, a stress-induced family of MHC-I-like proteins. These ligands are upregulated on stressed/infected cells and are not widely expressed on healthy adult tissue. NKG2D ligands have been widely studied as potential targets for ...
Phenotyping of Live Human PBMC using CyTOFTM Mass Cytometry
Authors: Michael D. Leipold and
Holden Maecker,
date: 01/20/2015,
view: 13143,
Q&A: 3
[Abstract] Single-cell analysis has become an method of importance in immunology. Fluorescence flow cytometry has been a major player. However, due to issues such as autofluorescence and emission spillover between different fluorophores, alternative techniques are being developed. In recent years, mass cytometry has emerged, wherein antibodies labeled with ...