K+ Release Assay and K+ Measurement in Oocyte Assay
Author: Hong Li,
date: 11/05/2020,
view: 831,
Q&A: 0
[Abstract] The Xenopus oocyte is a powerful system for the exogenous expression and functional characterization of plant membrane transport proteins. Until now, a number of potassium transporters and channels have been identified in oocytes expression system by the two-electrode voltage clamp technology. It is difficult to characterize K+ ...
Quantification of the Humidity Effect on HR by Ion Leakage Assay
Authors: Musoki Mwimba and
Xinnian Dong,
date: 04/05/2019,
view: 2866,
Q&A: 0
[Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
[Abstract] There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict ...