Quantification of the Humidity Effect on HR by Ion Leakage Assay
Authors: Musoki Mwimba and
Xinnian Dong,
date: 04/05/2019,
view: 1329,
Q&A: 0
[Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
[Abstract] There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict ...
Bacterial Intracellular Sodium Ion Measurement using CoroNa Green
[Abstract] The bacterial flagellar type III export apparatus consists of a cytoplasmic ATPase complex and a transmembrane export gate complex, which are powered by ATP and proton motive force (PMF) across the cytoplasmic membrane, respectively, and transports flagellar component proteins from the cytoplasm to the distal end of the growing flagellar structure ...
Putrescine Biosynthesis Inhibition in Tomato by DFMA and DFMO Treatment
[Abstract] This protocol can be used to inhibit the biosynthesis of polyamines, specifically putrescine, in tomato plants grown with NH4+ as a solely N source. In general, polyamines are positively charged small metabolites implicated in physiological processes, including organogenesis, embryogenesis, floral initiation and development, ...