Protocols in Current Issue
    A Protocol for Flavonols, Kaempferol and Quercetin, Staining in Plant Root Tips
    Author:  Nguyen Hoai Nguyen, date: 10/05/2020, view: 1249, Q&A: 0
    [Abstract] Flavonols are a subclass of flavonoids of the group of plant secondary metabolites. In planta, flavonols play various functions such as antioxidant and natural regulator of auxin polar transport. Many lines of evidence have shown that flavonols also contribute to human health in anti-oxidation, anti-inflammation, and even prevention some ...
    Isolation of Flavonoids from Piper delineatum Leaves by Chromatographic Techniques
    Authors:  Isabel L. Bazzocchi, Juan C. Ticona, Ignacio A. Jiménez and Ninoska Flores, date: 07/20/2016, view: 14960, Q&A: 0
    [Abstract] The genus Piper (Piperaceae) is widely distributed in the tropical and subtropical regions of the world, and species belonging to this genus are included in the Ayurvedic system of medicine and in folklore medicine of Latin America. Phytochemical investigations of Piper species have led to the isolation of several classes of ...
    A Bioassay Protocol for Quorum Sensing Studies Using Vibrio campbellii
    Authors:  Alberto J. Martín-Rodríguez and José J. Fernández, date: 07/20/2016, view: 8822, Q&A: 0
    [Abstract] Quorum Sensing (QS), or bacterial cell-to-cell communication, is a finely-tuned mechanism that regulates gene expression on a population density-dependent manner through the production, secretion and reception of extracellular signaling molecules termed autoinducers (AIs). Given that QS plays an important role in bacterial biofilm formation and ...
    Quantification of Anthocyanin Content
    Authors:  Masaru Nakata and Masaru Ohme-Takagi, date: 04/05/2014, view: 22749, Q&A: 2
    [Abstract] Anthocyanins are a class of flavonoids and important plant pigments. They attract insects to pollinate flowers, protect plants from UV irradiation, and act as antimicrobial agents against herbivores and pathogens. Biosynthesis of anthocyanin is stimulated by diverse developmental signals and environmental stresses including drought, wounding, ...
    Extraction of Coumarins from Leaves, Petioles, Stems and Roots of Ruta graveolens and Nicotiana benthamiana
    Authors:  Alain Hehn, Guilhem Vialart, Alexandre Olry and Frederic Bourgaud, date: 11/20/2012, view: 9748, Q&A: 0
    [Abstract] This method describes the extraction of coumarins and furanocoumarins from leaves of Ruta graveolens (a natural furanocoumarin) producer, and Nicotiana benthamiana.

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