Enzymatic Activity Assay for Invertase in Synechocystis Cells
Authors: Xiaoming Tan,
Kuo Song and
Xuefeng Lu,
date: 05/20/2018,
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[Abstract] Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be determined. All the methods for determination of the invertase activity ...
Observation of Pneumococcal Phase Variation in Colony Morphology
[Abstract] Streptococcus pneumoniae (pneumococcus) is an important human pathogen that causes pneumonia, meningitis, sepsis, and otitis media. This bacterium normally resides in the nasopharynx as a commensal, but sometimes disseminates to sterile sites of humans and causes local or systemic inflammation. This biphasic behavior of S. pneumoniae ...
Digestion of Peptidoglycan and Analysis of Soluble Fragments
Authors: Ryan E. Schaub and
Joseph P. Dillard,
date: 08/05/2017,
view: 8982,
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[Abstract] Peptidoglycan (murein) is a vital component of the cell wall of nearly all bacteria, composed of sugars linked by short peptides. This protocol describes the purification of macromolecular peptidoglycan from cultured bacteria and the analysis of enzyme-digested peptidoglycan fragments using high performance liquid chromatography (HPLC). Digested ...