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Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient

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Pneumatic Method to Measure Plant Xylem Embolism

Authors: Paulo R. L. Bittencourt, Luciano Pereira and Rafael S. Oliveira, date: 10/20/2018, view: 1716, Q&A: 0

[Abstract] Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning and triggering plant mortality. Measuring embolism with traditional hydraulic methods is both ...

Increasing the Membrane Permeability of a Fern with DMSO

Author: Marcelo Garcés, date: 06/20/2018, view: 1784, Q&A: 0

[Abstract] Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum fronds, we incubate fronds with 0.1% DMSO to test if this increases cell membrane permeability ...

Slow and Fast Desiccation of Single-cell Thick Fronds of Filmy Ferns

Author: Marcelo Garcés, date: 06/05/2018, view: 1605, Q&A: 0

[Abstract] Filmy ferns can desiccate and recover after rehydration to resume photosynthesis. Slow and fast desiccation rates were compared in filmy fern fronds to determine whether structural or physiological differences may occur between desiccation rates. Slow desiccation is considered to be more similar to natural conditions experienced by plants that ...

Measurement of Arabidopsis thaliana Plant Traits Using the PHENOPSIS Phenotyping Platform

Authors: Wojciech Rymaszewski, Myriam Dauzat, Alexis Bédiée, Gaëlle Rolland, Nathalie Luchaire, Christine Granier, Jacek Hennig and Denis Vile, date: 02/20/2018, view: 3573, Q&A: 0

[Abstract] High-throughput phenotyping of plant traits is a powerful tool to further our understanding of plant growth and its underlying physiological, molecular, and genetic determinisms. This protocol describes the methodology of a standard phenotyping experiment in PHENOPSIS automated platform, which was engineered in INRA-LEPSE ( ...

Monitoring Xylem Hydraulic Pressure in Woody Plants

Authors: Guillaume Charrier, Régis Burlett, Gregory Gambetta, Sylvain Delzon, Jean-Christophe Domec and François Beaujard, date: 10/20/2017, view: 4223, Q&A: 0

[Abstract] Xylem sap circulates under either positive or negative hydraulic pressure in plants. Negative hydraulic pressure (i.e., tension) is the most common situation when transpiration is high, and several devices have been developed to quantify it accurately (e.g., Scholander pressure chamber, psychrometers). However, a proper ...

Assessing Plant Tolerance to Acute Heat Stress

Authors: Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling, date: 07/20/2017, view: 5438, Q&A: 1

[Abstract] It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability of plants to acquire tolerance to high temperature stress. The hypocotyl elongation assay described ...

Forest GPP Calculation Using Sap Flow and Water Use Efficiency Measurements

Authors: Fyodor Tatarinov, Eyal Rotenberg, Dan Yakir and Tamir Klein, date: 04/20/2017, view: 4254, Q&A: 0

[Abstract] This is a protocol to evaluate gross primary productivity (GPP) of a forest stand based on the measurements of tree’s sap flow (SF), ¹³C derived water use efficiency (WUE), and meteorological (met) data. GPP was calculated from WUE and stomatal conductance (g_s), the later obtained from SF up-scaled from sampled trees to stand ...

Establishment of New Split-root System by Grafting

Authors: Xiangqiang Kong, Zhen Luo and Hezhong Dong, date: 02/20/2017, view: 4819, Q&A: 0

[Abstract] A new split-root system was used to simulate non-uniform salt, drought or nutrient deficiency stress in the root zone, in which the root system was divided into two or more equal portions. Here, we established a split-root system by grafting of cotton seedlings. In contrast to the conventional split-root, the main roots of the new system remained ...

A Method to Analyze Local and Systemic Effects of Environmental Stimuli on Root Development in Plants

Authors: Neil E. Robbins II and José Dinneny, date: 09/05/2016, view: 6037, Q&A: 0

[Abstract] Root development in vascular plants is innately tied to the environment. However, relatively little attention has been paid toward understanding the spatial scales at which the root perceives and responds to external stimuli. While some environmental signals elicit global responses that affect root system architecture, others may have more ...

Measurements of Proline and Malondialdehyde Content and Antioxidant Enzyme Activities in Leaves of Drought Stressed Cotton

Authors: Tianzi Chen and Baolong Zhang, date: 09/05/2016, view: 11397, Q&A: 2

[Abstract] Drought stress negatively affects cotton plant growth and induces various biochemical and physiological responses in cotton plants. Proline content and antioxidant enzymes are thought to be associated with maintaining the structure of cellular components or with protecting cellular function. Study of cotton plant responses towards drought stress ...

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