Plant Science

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    Protocols in Current Issue
    Biolistic Bombardment for Co-expression of Proteins Fused to YFP and mRFP in Leaf Epidermal Cells of Phaseolus vulgaris ‘Red Mexican’
    Author:  Malgorzata Lichocka, date: 01/05/2014, view: 11005, Q&A: 0
    [Abstract] Biolistic bombardment is based on coating of tungsten or gold particles with DNA and delivery of these “biobullets” into living plant cells under high pressure (Sudowe and Reske-Kunz, 2013). This method enables transient expression of a DNA construct encoding fusion of the protein of interest to a fluorescence protein e.g. GFP for ...
    Maize Embryo Transient Transformation by Particle Bombardment
    Author:  Matilde Jose-Estanyol, date: 08/20/2013, view: 7606, Q&A: 0
    [Abstract] Particle bombardment has been shown to be a useful method to study gene promoter regulatory elements by transient transformation of maize embryos with different constructions of gene promoters fused to a gene reporter. DNA to transfer is coated to high density gold microparticles and introduced into cells when accelerated by a helium pulse. This ...



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