Molecular Biology

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    Protocols in Current Issue
    Conjugation of Duplexed siRNN Oligonucleotides with DD-HyNic Peptides for Cellular Delivery of RNAi Triggers
    Authors:  Alexander S. Hamil, Khirud Gogoi and Steven F. Dowdy, date: 04/05/2016, view: 6031, Q&A: 0
    [Abstract] Despite the great promise that short interfering RNA (siRNA) induced RNAi responses hold as a therapeutic modality, due to their size (~15 kDa) and high negative charge (Bumcrot et al., 2006), siRNAs have no bioavailability and require a delivery agent to enter cells (Figure 1). TAT peptide transduction domain (PTD) has been developed as ...
    Post-crystallization Improvement of RNA Crystals by Synergistic Ion Exchange and Dehydration
    Authors:  Jinwei Zhang and Adrian R. Ferré-D’Amaré, date: 09/05/2015, view: 5848, Q&A: 0
    [Abstract] Compared to the recent dramatic growth in the numbers of genome-wide and functional studies of complex non-coding RNAs, mechanistic and structural analyses have lagged behind. A major technical bottleneck in the structural determination of large RNAs and their complexes is preparation of diffracting crystals. Empirically, a vast majority of such ...
    Synthesis of 5’ end-labeled RNA
    Author:  Harald Putzer, date: 07/20/2012, view: 10960, Q&A: 2
    [Abstract] 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not work on 5’ PPP RNA and require a 5’ P substrate. The activity of certain ...



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