Molecular Biology

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    Protocols in Current Issue
    Isolation and Transcriptomic Profiling of Single Myofibers from Mice
    [Abstract] Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells composing the organ contribute to the expression value detected for each gene with the loss of ...
    Generation of microRNA Sponge Library
    Author:  Sebastian Herzog, date: 04/20/2018, view: 3750, Q&A: 0
    [Abstract] This protocol describes the generation and functional validation of microRNA (miRNA) sponge or decoy constructs. When expressed from a strong promoter, these transcripts can sequester specific miRNA:RISC complexes, thereby resulting in a derepression of endogenous target mRNA. Hence, cells expressing such sponges display a partial or full miRNA ...
    Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets
    Authors:  Pornima Phatak and James M Donahue, date: 05/05/2017, view: 11983, Q&A: 1
    [Abstract] microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.



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