Molecular Biology

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    Protocols in Current Issue
    Organelle-associated rRNA Degradation
    Authors:  Jinliang Huang and Geng Wang, date: 06/05/2019, view: 2521, Q&A: 0
    [Abstract] Cytosolic rRNAs are highly dynamic and can be degraded under conditions such as apoptosis, starvation and magnesium depletion. The degradation is also related to their specific localization, as fractions of cytosolic ribosomes are localized on the surfaces of intracellular organelles, such as endoplasmic reticulum (ER) and mitochondria. Such ...
    RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells
    Authors:  Michal Domanski and John LaCava, date: 04/20/2017, view: 6200, Q&A: 0
    [Abstract] The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al., 2016]). The RNA exosome-associated EXOSC10 protein is a distributive, 3’-5’ exoribonuclease. ...
    Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells
    Authors:  Michal Domanski and John LaCava, date: 04/20/2017, view: 5784, Q&A: 0
    [Abstract] The RNA exosome complex plays a central role in RNA processing and regulated turnover. Present both in cytoplasm and nucleus, the exosome functions through associations with ribonucleases and various adapter proteins (reviewed in [Kilchert et al., 2016]). The following protocol describes an approach to purify RNA exosome complexes from ...



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