Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
Authors: Van T. Luu,
Melissa Stiebner,
Paula Emmerich Maldonado,
Sandra Valdés,
Didier Marín,
Gerardo Delgado,
Virginia Laluz,
Lin-Bo Wu,
Paul Chavarriaga,
Joe Tohme,
Inez H. Slamet-Loedin and
Wolf B. Frommer,
date: 09/05/2020,
view: 1404,
Q&A: 0
[Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
[Abstract] The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production of riboflavin (vitamina B2). In ...
Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale
[Abstract] Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
[Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
Authors: Tzemach Aouizerat,
Aren M. Maeir,
Yitzhak Paz,
Yuval Gadot,
Amir Szitenberg,
Sivan Alkalay-Oren,
Shunit Coppenhagen-Glazer,
Michael Klutstein and
Ronen Hazan,
date: 01/05/2020,
view: 2012,
Q&A: 0
[Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...
Protocol for Ribosome Profiling in Bacteria
Authors: Fuad Mohammad and
Allen R. Buskirk,
date: 12/20/2019,
view: 2734,
Q&A: 0
[Abstract] Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight into the mechanism and regulation of protein synthesis itself. First developed in yeast, ribosome ...