Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale
[Abstract] Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of the biochemistry and physiology of these bacteria has been limited by a lack of genetic ...
CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
[Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
Authors: Tzemach Aouizerat,
Aren M. Maeir,
Yitzhak Paz,
Yuval Gadot,
Amir Szitenberg,
Sivan Alkalay-Oren,
Shunit Coppenhagen-Glazer,
Michael Klutstein and
Ronen Hazan,
date: 01/05/2020,
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[Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...
Protocol for Ribosome Profiling in Bacteria
Authors: Fuad Mohammad and
Allen R. Buskirk,
date: 12/20/2019,
view: 1708,
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[Abstract] Ribosome profiling provides information on the position of ribosomes on mRNA on a genomic scale. Although this information is often used to detect changes in gene expression under different conditions, it also has great potential for yielding insight into the mechanism and regulation of protein synthesis itself. First developed in yeast, ribosome ...
Quantification of HIV-2 DNA in Whole Blood
[Abstract] Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, ...