Assessing Yeast Cell Survival Following Hydrogen Peroxide Exposure
Authors: Khoa Tran and
Erin M. Green,
date: 01/20/2019,
view: 767,
Q&A: 0
[Abstract] In the presence of oxidative stress, cellular defense systems that can detoxify reactive oxygen species are activated through multiple signaling cascades and transcriptional reprogramming. The budding yeast Saccharomyces cerevisiae has served as an excellent model for genetically-identifying factors important for the response to oxidative ...
Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone
Authors: Jeffrey W. Perry and
Andrew W. Tai,
date: 08/20/2018,
view: 963,
Q&A: 0
[Abstract] Protein tagging is a powerful method of investigating protein function. However, modifying positive-strand RNA virus proteins in the context of viral infection can be particularly difficult as their compact genomes and multifunctional proteins mean even small changes can inactivate or attenuate the virus. Although targeted approaches to ...
CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
[Abstract] Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous recombination resulting in the desired modified virus is a rare event. We recently reported the use of ...
Single and Multiplexed Gene Editing in Ustilago maydis Using CRISPR-Cas9
[Abstract] The smut fungus Ustilago maydis is an established model organism for elucidating how biotrophic pathogens colonize plants and how gene families contribute to virulence. Here we describe a step by step protocol for the generation of CRISPR plasmids for single and multiplexed gene editing in U. maydis. Furthermore, we describe the ...
Induction of Natural Competence in Genetically-modified Lactococcus lactis
[Abstract] Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence by regulating the expression of the comX gene by using a nisin-inducible promoter in an L. ...